ABSTRACT
This study was carried out to evaluate the renal
protective effect of a combined ethanol extract of Andrographispaniculata,
Mucunapruriens and Ficussur (AMFICUS) ofalloxan-induced diabetic rats. The
acute toxicity effect ofAMFICUS was evaluated with twenty-one (21) male albino
rats while forty-two (42) male albino rats were used for the evaluation ofthe
renoprotective effect of AMFICUS on alloxan-induced diabetic rats. The
forty-two rats were randomly selected into seven groups containing equal number
of rats (n = 6). Group 1 was the normal control that received 2 mL/kg normal
saline and groups 2-6 were alloxan-induced diabetic rats. The rats were induced
diabetic by the intraperitoneal administration of a single dose (160 mg/kg) of
alloxan dissolved in normal saline and allowed to stay for 72 h for onset
ofdiabetes. Alloxan-induced rats having blood glucose level above 200 mg/dl
were considered diabetic and used for the study. The diabetic induced rats were
classified as diabetic control, diabetic rats + 3 mg/kg Glibenclamide, diabetic
rats + 200 mg/kg AMFICUS, diabetic rats + 400 mg/kg AMFICUS, and diabetic rats
+ 800 mg/kg AMFICUS. The groups 7 rats served as AMFICUS control that received
800 mg/kg AMFICUS only. The study was conducted following standard laboratory
procedures. The results ofthe acute toxicity study of AMFICUS indicated that it
has LD50 above 5000 mg/kg. The results of renaprotective study of AMFICUS
indicated that the diabetic control rats exhibited significantly (P<0.05)
elevated serum urea, creatinine, sodium, potassium, chloride and bicarbonate
ion concentrations compared with the normal control and rats administered 800
mg/kg AMFICUS only, respectively. The alterations in the serum urea,
creatinine, sodium, potassium, chloride, and bicarbonate ion concentrations
were significantly reversed in the diabetic rats treated with different doses
ofAMFICUS mA Glibenclamide respectively compared with the normal control. The
renal sections of diabetic control rats and diabetic rats treated with 200 and
400 mg/kg AMFICUS respectively, displayed multifocal areas oftubular
degenerations and necrosis relative to the renal histomorphology of the normal
control. Contrarily, the renal histomorphology of diabetic rats treated with
800 mg/kg AMFICUS, and rats administered 800 mg/kg AMFICUS only respectively
showed normal histomorphology compared to the diabetic control. The findings
from this study show that AMFICUS possesses renoprotective effect against renal
disorders associated with alloxan-induced diabetes in rats and suggest it could
play a critical role in the management of diabetes.
NWOYE, N (2024). Renoprotective Study Of A Combined Ethanol Extracts Of Andrographispaniculata, Mucuna Pruriens And Ficus Sur (Amficus) On Alloxan-Induced Diabetic Rats:- Nwoye, Uchechukwu F. Mouau.afribary.org: Retrieved Dec 13, 2024, from https://repository.mouau.edu.ng/work/view/renoprotective-study-of-a-combined-ethanol-extracts-of-andrographispaniculata-mucuna-pruriens-and-ficus-sur-amficus-on-alloxan-induced-diabetic-rats-nwoye-uchechukwu-f-7-2
NWOYE, NWOYE. "Renoprotective Study Of A Combined Ethanol Extracts Of Andrographispaniculata, Mucuna Pruriens And Ficus Sur (Amficus) On Alloxan-Induced Diabetic Rats:- Nwoye, Uchechukwu F" Mouau.afribary.org. Mouau.afribary.org, 13 Nov. 2024, https://repository.mouau.edu.ng/work/view/renoprotective-study-of-a-combined-ethanol-extracts-of-andrographispaniculata-mucuna-pruriens-and-ficus-sur-amficus-on-alloxan-induced-diabetic-rats-nwoye-uchechukwu-f-7-2. Accessed 13 Dec. 2024.
NWOYE, NWOYE. "Renoprotective Study Of A Combined Ethanol Extracts Of Andrographispaniculata, Mucuna Pruriens And Ficus Sur (Amficus) On Alloxan-Induced Diabetic Rats:- Nwoye, Uchechukwu F". Mouau.afribary.org, Mouau.afribary.org, 13 Nov. 2024. Web. 13 Dec. 2024. < https://repository.mouau.edu.ng/work/view/renoprotective-study-of-a-combined-ethanol-extracts-of-andrographispaniculata-mucuna-pruriens-and-ficus-sur-amficus-on-alloxan-induced-diabetic-rats-nwoye-uchechukwu-f-7-2 >.
NWOYE, NWOYE. "Renoprotective Study Of A Combined Ethanol Extracts Of Andrographispaniculata, Mucuna Pruriens And Ficus Sur (Amficus) On Alloxan-Induced Diabetic Rats:- Nwoye, Uchechukwu F" Mouau.afribary.org (2024). Accessed 13 Dec. 2024. https://repository.mouau.edu.ng/work/view/renoprotective-study-of-a-combined-ethanol-extracts-of-andrographispaniculata-mucuna-pruriens-and-ficus-sur-amficus-on-alloxan-induced-diabetic-rats-nwoye-uchechukwu-f-7-2