ABSTRACT

Soil is the major repository of microorganism that clinically useful antibiotics have been isolated for several group of microorganism including bacteria (Streptomyces sp, Bacillus sp). Bacillus and Actinomycetes are the most abundant microorganism present in Soil and inhibit the growth of other microorganisms. Present in soil and inhibit the growth of other microorganism. In this study 5 Soil samples from different ecological sites were obtained and Cultured in microbiological media to isolate microorganisms. Ten fold serial dilution of the Soil samples were prepared and inoculated on nutrient agar and Saboraud Dextrose agar. A total of 15 Isolated were recovered. Ten of the total isolated were sub cultured on nutrient agar and incubated at 37oc for 24hrs. Out of these ten isolates four are Micrococcus, four Bacillus species and two Actinomycetes were obtained. Five different isolates was tested against four test organisms. Namely: Staphylococcus aureus, Pseudomonas aeruginosa, Mycobacterium semgmatis and Escherichia coli   for antibacterial activity. Out of the five isolates, only two (NDDCfemale Hostel) exhibitory effect against one of the test organisms (Pseudomonas aeruginosa) with zones of inhibition of 9mm and 13mm respectively. The remaining isolates showed no antimicrobial activity against the test organisms. Therefore, it is confirmed that soil samples for microorganisms collected within the University Premises of Umudike were found to be potential source of OR one of the isolates (NDDC Hostel) that is Actinomyces was able to inhibit growth of Staphylococcus aureus with a clear inhibition range with a clear inhibition range 0-14mm, which indicates an antibacterial activity against S. aureus while others showed no antimicrobial activity against the test organisms. This shows that the isolation of (NDDC Hostel) from this study area may contribute to the discovery of with further extraction and purification of the isolate.