ANTIFUNGAL EFFECTS OF BACTERIA SPECIES ISOLATED FROM THE RHIZOPHERE OF GINGER (Zingiber officinale)

NKIRUKA PEACE MOUAU/MCB/14/18789 | 34 pages (7242 words) | Projects
Microbiology | Co Authors: OHAZURUME

ABSTRACT

The project studied the antifungal effects of bacteria species isolated from the Rhizosphere of ginger plant. Seven species of bacteria were isolated from five rhizospheric soils of ginger plants with varying levels of occurrence including a species of Bacillus (100%), Pseudomonas sp (100%), Staphylococcus sp (100%), Proteus (60%), Actinomyces (100%), Escherichia coli (20%) and lactobacillus (40%). Antifungal activity was tested on five chosen fungal species which included Aspergillus sp, Penicilliltm sp, Fusarium sp, Rhizopus sp and Yeast sp. The results obtained revealed that three microbial isolates, Bacillus sp, Actinomyces and Pseudomonas were active against the fungi but to varying extents. Bacillus showed the highest antifungal potency with inhibition zones diameters that ranged from 13.67mm to 22.67mm while Pseudomonas had the least (1.67mm to 17.67mm). The inhibition of Actinomyces was in the range of 14.33mm to 17.33mm. However, the activities of the bacteria isolates against the fungi were lower than that of commercial antifungal agent, mancozade. Notwithstanding, the bacteria isolates showed high relative potencies when compared with the standard antifungal drug. The relative potency of Bacillus isolate was between 64.09% to 88.31% that of Pseudomonas was in the range of 54.71% to 61.04% while that of the Actinomyces was in the range of 55.82 to 73.47%. There were significant variation in the antifungal effect of the ginger rhizospheric bacterial isolates on the test fungi. Therefore some bacteria species were isolated from the rhizosphere of ginger that have antifungal tendencies.


TABLE OF CONTENTS

Titles                                                                                  Page

Title Page                                                                  i

Certification                                                              ii

Dedication                                                                  iii

Acknowledgements                                                    iv

Table of Contents                                                      v

List of Tables                                                            vii

Abstract viii 

CHAPTER ONE

1.0                                      INTRODUCTON                                         1

1.1                                      Introduction                 1

1.2                                      Aims                                                                     3

1.3                                      Objectives                                                                3

CHAPTER TWO

2.0                                      LITERATURE REVIEW                                             4

2.1                                      Rhizosphere Plant                                                        4

2.2                                      Beneficial Micro Organism in Plant Rhizosphere         5

2.3                                      Bacteria Association with Rhizosphere                         6

2.4                                      Microbial Ecology of Rhizosphere                              7

2.5                                      Control of Fungi Pathogen                                           8

2.6                                      Microorganism Found in the Rhizosphere of Ginger    8

2.7                                      Ginger As An Antioxidant                                           9

2.7.1                                    Antioxidant Studies of Ginger Extracts                       9


CHAPTER THREE

3.0                                           MATERIALS AND METHODS                               10

3.1                                      Materials                                                                      10 

3.1.1                                    Sources of Materials    10

3.2                                      Methods                                                                    10 

3.2.1                                     Sample and Media Preparation                                    10

3.2.2                                   Media Preparation                                                    10

3.2.3                                   Preparation of Soil Sample                                        11 

3-3                                     Isolation of Rliizospheric Bacteria                              12 

3-3.1     ,                             Characterization of bacteria isolates                            12

3.3.2                                   Colony features (morphology)                                   12

3.3.3                                     Microscopic characteristics                                         12

3.3.4                                     Biochemical characteristics                                         13

3.3.5                                     Carbohydrate (sugar) utility tests                                 13

 3.4                                         Identification of bacteria isolates                                13

CHAPTER FOUR

4.0                                      RESULTS                                                              14

CHAPTER FIVE

5.0                                       DISCUSSION, CONCLUSION AND

RECOMMENDATION                                             21

5.1                                          Discussion                                                               21

5.2                                        Conclusion                                                            23

5.3                                        Recommendation                                                   23

REFERENCES


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APA

NKIRUKA, M (2020). ANTIFUNGAL EFFECTS OF BACTERIA SPECIES ISOLATED FROM THE RHIZOPHERE OF GINGER (Zingiber officinale). Mouau.afribary.org: Retrieved Nov 14, 2024, from https://repository.mouau.edu.ng/work/view/antifungal-effects-of-bacteria-species-isolated-from-the-rhizophere-of-ginger-zingiber-officinale

MLA 8th

MOUAU/MCB/14/18789, NKIRUKA. "ANTIFUNGAL EFFECTS OF BACTERIA SPECIES ISOLATED FROM THE RHIZOPHERE OF GINGER (Zingiber officinale)" Mouau.afribary.org. Mouau.afribary.org, 29 Apr. 2020, https://repository.mouau.edu.ng/work/view/antifungal-effects-of-bacteria-species-isolated-from-the-rhizophere-of-ginger-zingiber-officinale. Accessed 14 Nov. 2024.

MLA7

MOUAU/MCB/14/18789, NKIRUKA. "ANTIFUNGAL EFFECTS OF BACTERIA SPECIES ISOLATED FROM THE RHIZOPHERE OF GINGER (Zingiber officinale)". Mouau.afribary.org, Mouau.afribary.org, 29 Apr. 2020. Web. 14 Nov. 2024. < https://repository.mouau.edu.ng/work/view/antifungal-effects-of-bacteria-species-isolated-from-the-rhizophere-of-ginger-zingiber-officinale >.

Chicago

MOUAU/MCB/14/18789, NKIRUKA. "ANTIFUNGAL EFFECTS OF BACTERIA SPECIES ISOLATED FROM THE RHIZOPHERE OF GINGER (Zingiber officinale)" Mouau.afribary.org (2020). Accessed 14 Nov. 2024. https://repository.mouau.edu.ng/work/view/antifungal-effects-of-bacteria-species-isolated-from-the-rhizophere-of-ginger-zingiber-officinale

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