Antibiotic Susceptibility Profile Of Extended Spectrum Of Beta Lactamase Of E.Coli Isolated From Poultry Environment In Three Poultry Farms.:- Iloh, Njideka F

ABSTRACT

 The aim ofthis study is to assess the Extended Spectrum Beta Lactamase profile of Escherichia coli from soil of different poultry farms. Ten (10) samples each were collected from three . different poultry farms in Umuahia, Abia State and the samples were collected aseptically into a sterile plastic bag and labeled accordingly. They were transported immediately to the laboratory for microbiological analysis. A ten fold serial dilution of each ofthe fecal samples was prepared using sterile distilled water. 0.1 mL aliquots of the serially-diluted samples (10'2and 10’4) was spread-plated on the medium. The sensitivity pattern of the isolate revealed that Ceftazidime (60%), was more sensitive to the isolate followed by, Ceftizoxime (56%), and Cefotaxime (56%) while Amoxicillin (60%) was resistance to the isolate followed by Ofloxacin (56%),, Ampicillin (56%), Gentamicin (53.3%) while Chloramphenicol (50%) was less resistance to the isolate. The Extended Spectrum P-lactamase profile of E.coli from different poultry farms. The results showed that Umuahia had more prevalence of ESBL producing E.coli (83%) when compared with Mouau (75%) and Umuariaga (JI Escherichia coli is the most prevalent genus of ESBL resistant enterobacteria colonizing poultry animals within the study area. The study shows that chicken reared in the study area are potential reservoirs and disseminators of ESBL-resistant enterobacteria and genes encoding for ESBLs production thus, the study recommended that ESBL producing organisms should be identified quickly so that appropriate antibiotic usage and infection control measures can be implemented.