ABSTRACT
Biofilm in waste water treatment system was evaluated. Slime (film) formers were isolated from water samples collected from treated waste water; from brewery and bottling company in Aba. Result obtained from the adherence slip culture show the presence of five bacteria isolates with ability to adhere strongly onto the cover slip in water. The isolates and occurance were; Staphylococcus, 100%, Bacillus, 100%, Pseudomonas 80%, E.coli 40% and Listeria 50%. Further tests for confirmation of biofilm production was done using the congo red agar colorimetric method. Staphylococcus, Bacillus, and Pseudomonas showed positive biofilm production while E.coli and Listeria did not produce slime in the culture. Also present of physico-chemical properties of the waste water samples indicates low quality with high BOD of 9.7mg/l to 22.5mg/l in the treated water bottling company waste water and from 9.67mg/l to 13.7mg/l. The COD range was 4.61mg/l to 8.06mg/l and from 2.75mg/l to 5.66mg/l in the two waste waters respectively. The pH ranged from 5.33 to 5.79 and from 5.43 to 6.23 in the water samples. The more variations in Temperature of the waters over the days as well as in the Total Solids and Total Suspended Solids content. The water was considered to be polluted by organic pollutant and unsuitable for domestic use as the biofilm can cause deliteries effects on utensils including corrosion and possible infections.
TABLE OF CONTENTS
Title page i
Certification ii
Dedication iii
Acknowledgement iv
Table of Contents v
List of Tables vii
List of Figures viii
Abstract ix
CHAPTER ONE
1.1 Introduction 1
1.2 Advantages and disadvantages of biofilm application 2
1.3 Aims and objectives 3
CHAPTER TWO - LITERATURE REVIEW 4
2.1 Wastewater and Its Treatment 4
2.1.1 Wastewater Components 5
2.2 Biofilms 7
2.2.1 Development of formation of biofilm 8
2.2.2 Biofilm system in Wastewater 11
2.2.3 Comparison between suspended and fixed film 12
2.2.4 Sludge mechanisms 13
2.2.5 Wastewater and anaerobic treatment 14
CHAPTER THREE - MATERIALS AND METHOD 16
3.1 Sources of Material 16
3.2 Sample and Media Preparation. 16
3.2.1 Sample and Sampling 16
3.2.2 Media Preparations 16
3.3 Determination of Bacterial Load 17
3.4 Isolation of Biofilm Producing Bacteria 18
3.4.1 Characterisation and Identification of Biofilm suspect isolates 19
3.5 Determination of physiochemical properties of waste water samples. 20
3.5.1 Determination of temperature 20
3.5.2 Determination of pH 20
3.5.3 Determination of total and suspended solids. 20
3.5.4 COD determination 21
3.5.5 Determination of BOD 21
3.6 Determination of Biofilm formation of Isolates 22
CHAPTER FOUR – RESULTS 24
CHAPTER FIVE – DISCUSSION, CONCLUSION AND RECOMMENDATION 30
5.1 Conclusion 31
5.2 Recommendation 31
References
Appendixes
DORIS, M (2020). EVALUATION OF BIOFILM IN WASTEWATER TREATMENT SYSTEM. Mouau.afribary.org: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/evaluation-of-biofilm-in-wastewater-treatment-system
MOUAU/11/19793, DORIS. "EVALUATION OF BIOFILM IN WASTEWATER TREATMENT SYSTEM" Mouau.afribary.org. Mouau.afribary.org, 04 May. 2020, https://repository.mouau.edu.ng/work/view/evaluation-of-biofilm-in-wastewater-treatment-system. Accessed 24 Nov. 2024.
MOUAU/11/19793, DORIS. "EVALUATION OF BIOFILM IN WASTEWATER TREATMENT SYSTEM". Mouau.afribary.org, Mouau.afribary.org, 04 May. 2020. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/evaluation-of-biofilm-in-wastewater-treatment-system >.
MOUAU/11/19793, DORIS. "EVALUATION OF BIOFILM IN WASTEWATER TREATMENT SYSTEM" Mouau.afribary.org (2020). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/evaluation-of-biofilm-in-wastewater-treatment-system