ABSTRACT
The antimicrobial effectiveness of diluted antiseptics and the health risks that may be associated with any surviving pathogens were investigated. This experimental study was conducted using Kirby Bauer’s disk diffusion method of antimicrobial susceptibility test. Dettol, Savlon, Septol, Purit and NCP are five different kinds of antiseptics which have been widely used in common laboratories, hospitals, homes, barbing shops, etc. In this study, a compared experiment on these five antiseptics effectiveness was conducted against Staphylococcus aureus and Escherichia coli. Different concentrations of antiseptics (20%, 40%, 60%, 80% and 100%) were used on both organisms. Differences in concentrations tested was because, the original concentrations of the antiseptics differs. After 24 hours of incubation at 37 0C, the results showed that all the antiseptics inhibited the growth of the test organism in their concentrated forms. The diameter of zone of inhibitions were measured by using a ruler in millimeters, using different concentrations, their efficacies varied. The results showed that 60% to 80% Dettol and Savlon had the best and ideal efficiency against both test organisms, while NCP showed least sensitivity. 60% to 80% concentrations gave the highest effect on Staphylococcus aureus as compared with Escherichia coli.
TABLE OF CONTENTS
Cover page i
Title page ii
Certificate Page iii
Dedication iv
Acknowledgements v
Table of Contents vi
List of Tables ix
List of Figures x
Abstract xi
CHAPTER ONE: Introduction 1
1.1 Aims and Objectives 3
CHAPTER TWO: Literature Review 4
2.1 History of Antiseptics 5
2.3 Sources of Contamination of Living Tissues and Surfaces 8
2.4 Types of Antiseptics 9
2.5 Properties of an Antiseptics 15
2.6 General Features of Antiseptics 15
2.7 Factors Influencing the Efficacy of Antiseptics 17
2.8 Mechanism of Actions of Antiseptics Against Bacteria 18
2.9 Resistant Action of Bacteria 19
2.10 Uses of Antiseptics 19
2.11 Precautions when using Antiseptics 20
2.12 General Guidelines in the use of Antiseptics 21
2.13 General Features of the Test Organisms 22
2.13.1 Staphylococcus aureus 22
2.13.2 Escherichia coli 26
CHAPTER THREE: Materials And Methods 30
3.1 Study Area 30
3.2 Study Design 30
3.3 Collection of Antiseptics 30
3.4 Collection of Bacterial Samples 30
3.5 Sterilization of Materials and Preparation of Media 31
3.6 Serial Dilution and Inoculation of Bacterial Samples 31
3.7 Identification Tests 32
3.8 Serial Dilution of Antiseptics and Antimicrobial test 33
CHAPTER FOUR: Results 35
CHAPTER FIVE: Discussion, Conclusion and Recommendation 44
5.1 Discussion 44
5.2 Conclusion 45
5.3 Recommendations 46
REFERENCES 47
AHUWA, A (2020). Comparative Study Of Antimicrobial Strength Of Five Major Antiseptics Used In Umuahia, Abia State. Mouau.afribary.org: Retrieved Nov 30, 2024, from https://repository.mouau.edu.ng/work/view/comparative-study-of-antimicrobial-strength-of-five-major-antiseptics-used-in-umuahia-abia-state
AHUWA, AHUWA. "Comparative Study Of Antimicrobial Strength Of Five Major Antiseptics Used In Umuahia, Abia State" Mouau.afribary.org. Mouau.afribary.org, 03 Jun. 2020, https://repository.mouau.edu.ng/work/view/comparative-study-of-antimicrobial-strength-of-five-major-antiseptics-used-in-umuahia-abia-state. Accessed 30 Nov. 2024.
AHUWA, AHUWA. "Comparative Study Of Antimicrobial Strength Of Five Major Antiseptics Used In Umuahia, Abia State". Mouau.afribary.org, Mouau.afribary.org, 03 Jun. 2020. Web. 30 Nov. 2024. < https://repository.mouau.edu.ng/work/view/comparative-study-of-antimicrobial-strength-of-five-major-antiseptics-used-in-umuahia-abia-state >.
AHUWA, AHUWA. "Comparative Study Of Antimicrobial Strength Of Five Major Antiseptics Used In Umuahia, Abia State" Mouau.afribary.org (2020). Accessed 30 Nov. 2024. https://repository.mouau.edu.ng/work/view/comparative-study-of-antimicrobial-strength-of-five-major-antiseptics-used-in-umuahia-abia-state