Study Of Microbial Diversity In Human Stool Sample

ABSTRACT

The present study aimed at comparing the microbiota of stool of healthy patients, and the microbiota of the diarrhoeic stool of patients with gastro intestinal tract infection. A total of 20 sample were used for the study, of which 7 were diagnosed of gastro intestinal enteritis in Abia state specialist hospital A.S.S.H  Umuahia  Aba road while the remaining were samples collected from ulcerative patient diagnosed of helicobacter pylori stool sample, while the remaining were 6 formed stool sample collected from healthy individuals in MOUAU. All the sample were cultured on salmonella-shigella agar, MacConckey agar for 24hours at 370C. Gram staining was done and biochemical test was also carried out in the pure culture out of 20 sample, 17 were positive. These were 6 salmonella spp, 7 Shigella spp. and Echerichia coli were bacteria pathogens responsible for diarrhea from the gastrointestinal patients. At a favourable condition, these microorganisms establish themselves in the host system, thereby posing a lot of clinical symptoms such as diarrhea, vomiting, abdominal cramp, headache, nausea etc which if not properly treated can lead to death. The best means of handling food borne infection and intoxication is by queuing into the measures of preventing it as detailed in chapter of this work.