PREVALENCE AND BACTERIAL SPECIES ASSOCIATED WITH GASTROENTERITIS, A CASE STUDY WITH STUDENTS IN MICHAEL OKPARA UNIVERSITY OF AGRICULTURE UMUDIKE.

ABSTRACT

A study on prevalence and bacterial species associated with gastroenteritis among students in Michael Okpara University of Agriculture was conducted. Fifty stool samples from students that indicated cases of gastroenteritis from the questionnaire were examined. The stool samples were inoculated using the streak plate method. The following bacteria were isolated, Escherichia coli, salmonella species and Shigella species. Eschericha coli had the highest frequency of 54.2%, followed by Salmonella species (29.2%) while the least occurrence was recorded against Shigella species (16.7%). More cases were observed in females (52%) than in males (48%). Also, those living in the school hostel had more cases (66%) than those living off campus (34%). Effective sanitary condition should be maintained among students to avoid gastroenteritis which is a leading cause of morbidity that interferes with their academics.  

TABLE OF CONTENT

Cover page ﾿

Title page ﾿ i

Certification ﾿ ii

Dedication ﾿ iii

Acknowledgement ﾿ iv

Table of Contents ﾿ v 

List of Tables ﾿ vii

Abstract ﾿ viii

CHAPTER ONE ﾿ 1

1.0 ﾿        Introduction ﾿ 1

1.1        Aim of research ﾿ 3

CHAPTER TWO ﾿ 4

2.0 ﾿        Literature review ﾿ 4 

2.1 ﾿ Escherichia coli ﾿ 5 

2.2 ﾿ Sailmonella species ﾿ 7

2.3 ﾿ Campylobacter species ﾿ 7 

2.4 ﾿ Shigella species ﾿ 8 

2.5 ﾿ Aeromonas species ﾿ 9

2.6 ﾿ Yersinia species ﾿ 10

CHAPTER THREE ﾿ 11

3.0 ﾿        Materials and methods ﾿ 11

3.1 ﾿ Study area ﾿ 11

3.2 ﾿ Collection of samples ﾿ 11

3.3 ﾿ preparation of culture media ﾿ 11

3.4 ﾿ Isolation of bacteria species associated with gastroenteritis ﾿ 12

3.5 ﾿ Morphological examination of the isolates ﾿ 12

3.6 ﾿ Biochemical identification of isolates ﾿ 12

3.6.1 ﾿ Motility test ﾿ 12 

3.6.2 ﾿ Sugar fermentation test ﾿ 13 

3.6.3 ﾿ IMViC tests ﾿ 13 

3.6.4 ﾿ Urease test ﾿ 14

3.6.5 ﾿ Catalase test ﾿ 15

3.6.6 ﾿ Oxidase test ﾿ 15

CHAPTER FOUR ﾿ 16

4.0       Results ﾿ 16

CHAPTER FIVE ﾿ 20

5.0 ﾿      Discussion, conclusion and recommendation ﾿ 20

5.1 ﾿ Discussion ﾿ 20

5.2 ﾿ Conclusion ﾿ 21

5.3 ﾿ Recommendation ﾿ 22

References ﾿ 23

Appendix ﾿ 28