Phytochemical Analysis And Antimicrobial Activity Of Guava (Psidium Guajava) Leaves On Methicilin Resistant Staphylococcus Aureus And Escherichia Coli

ABSTRACT

The antimicrobial activity of the ethanolic and aqueous crude leaf extracts of Guava (Psidium guajava) leaf was evaluated against two clinical isolates namely Methicillin resistant Staphylococcus aureus (MRSA) and Escherichia coli using Agar disc diffusion technique. The result obtained revealed that the ethanolic extract showed the highest zone of inhibition (20mm) against Escherichia coli  at 400mg/ml concentration, and also showed the lowest zone of inhibition (11mm) against Methicillin resistant Staphylococcus aureus at 100mg/ml concentration. The phytochemical constituent of the Guava leaves identified were Alkaloids, Crude protein, Fat, Phenol, Saponins and Tannins. The MIC of Psidium guajava extract of Esherichia coli and Methicillin resistant Staphylococcus aureus are 25mg/ml and 12.5mg/ml respectively. The antimicrobial activity of the ethanolic and aqueous solvent extract against known  aetiologic agents of diseases traditionally treated with Psidium guajava provide justification of the use of the herb in traditional medicinal practice in Nigeria.