ISOLATION OF FUNGI FROM HAIR BARBING EQUIPMENT USED IN UMUAHIA

ABSTRACT

There is a growing concern that hair barbing equipments could serve as a potential source of fungal infections. This project work was conducted to isolate and identify fungi from equipment used in barbing salons in Umuahia, Abia State. Five (5) salons were visited and a total of fifteen (15) samples were collected from all the main equipment (3 samples each of clippers, combs, and scissors) used in the salon and cultured on Sabouraud’s Dextrose Agar (SDA). The results obtained showed the presence of dermatophytes which includes Microsporum audouinii and Trichophyton Species. On clipper, M. audouinii was 100%, 80% on scissors and 80% on comb and it has the highest number of occurrence while Trichophyton species was not isolated from clipper but occurred 20% on scissors and 20% on comb. It was observed that though some forms of sterilization are practiced in the salon, not all equipments are subjected to proper sterilization.

TABLE OF CONTENTS

Title page ﾿ i

Certification ﾿ ii

Dedication ﾿ iii

Acknowledgement ﾿ iv

Table of Contents ﾿ v

List of Tables ﾿ vii

Abstract ﾿                                                                                                                          viii

CHAPTER ONE

1.0 ﾿ Introduction ﾿ 1

1.1 ﾿ ﾿ Aim ﾿ 3

1.2 ﾿   ﾿ Objectives ﾿ 3

CHAPTER TWO

2.0 ﾿ Literature Review ﾿ 4

2.1 ﾿ Dermatophytes ﾿ 4

2.1.1 ﾿ Microsporum (Microsporum audouinii) ﾿ 5

2.1.2  ﾿ Trichophyton (Trichophyton rubrum) ﾿ 6

2.1.3  ﾿ Epidermophyton ﾿ 8

2.2  ﾿ Diseases Associated with the Scalp Caused by Dermatophytes ﾿ 8

2.2.1 ﾿ Tinea capitis ﾿ 8

2.2.2 ﾿ Pathology ﾿ 9

2.2.3  ﾿ Tinea corporis ﾿ 10

2.2.4 ﾿ Tinea versicolor ﾿ 11 ﾿

2.2.5  ﾿ Pityriasis versicolor ﾿ 11

2.3 ﾿ Treatment  ﾿ 12

2.4 ﾿ Infection Control Measures ﾿ 13

CHAPTER THREE

3.0 ﾿ Materials and Methods ﾿ 15

3.1 ﾿ Study Area ﾿ 15

3.2 ﾿ Sample Collection ﾿ 15

3.3 ﾿ Media Preparation ﾿ 15

3.4 ﾿ Isolation of Fungi ﾿ 16

3.4.1 ﾿ Identification of Fungi ﾿ 16

3.4.2 ﾿ Macroscopy ﾿ 16

3.4.3 ﾿ Microscopy ﾿ 16

CHAPTER FOUR

4.0 ﾿ Results ﾿ 17

CHAPTER FIVE

5.0    ﾿ Discussion, Conclusion and Recommendations ﾿ 20

5.1 ﾿ Discussion ﾿ 20

5.2 ﾿ Conclusion ﾿ 21

5.3 ﾿ Recommendations ﾿ 21

References

﾿ Appendix