Isolation, Characterization And Identification Of Bacteria From Organic Waste

ABSTRACT

The isolation, identification and characterization of bacteria from organic waste were carried out. Sawdust waste (plant organic waste source) and abattoir waste water (animal organic waste source) samples were collected. The media used were nutrient agar for total aerobic plate count, McConkey agar for coliform count and cellulolytic media for cellulolytic count. The pour plate method was employed. Colonial morphology, gram staining and biochemical tests were used for the identification of the bacteria. The total aerobic plate for the sawdust waste ranged from 2.33 x 107 to 8.2 x 106, coliform count ranged from 7.6 x 106to 1.7 x 106Cfu/g and cellulolytic count ranged from 4.7 x 106 to 2.6 x 106Cfu/g while for abattoir waste water, total aerobic plate count, coliform count and cellulolytic count ranged from 1.03 x 107 to 7.1 x 106Cfu/ml, 5.8 x 106 to 1.2 x 106Cfu/ml and 3.7 x 106 to 1.3 x 106Cfu/ml respectively. The bacteria isolated from the sawdust waste sample were Pseudomonas spp., Bacillus spp., Escherichia coli, Staphylococcus spp., klebsiella spp., Micrococcus spp., and Cellulomonas spp. While for the abattoir waste water, samples were Streptococcus spp., Pseudomonas spp., Staphylococcus aureus, Bacillus spp., Micrococcus spp.,Vibrio cholera, klebsiella spp., and Escherichia coli. The study concludes that microorganisms occur in a large number and variety in organic wastes.

TABLE OF CONTENTS

Certification ﾿       ﾿ i

Dedication ﾿           ﾿ ii

Acknowledgement ﾿              iii

Table of Contents ﾿              iv

List of Tables ﾿             ﾿ vi

Abstract ﾿             ﾿ vii

CHAPTER ONE

1.0 ﾿ Introduction ﾿     1   

1.1 ﾿ Justification of study ﾿                 2

1.2 ﾿ General objective of the study ﾿     3

1.3 ﾿ Specific objectives of the study ﾿     3

CHAPTER TWO

2.0 ﾿ Literature review ﾿     5

2.1 ﾿ Organic wastes    ﾿     5

2.2 ﾿ Sources of organic waste        ﾿     5 

2.2.1 ﾿ Plant source ﾿     5

2.2.2 ﾿ Animal source ﾿     5

2.3 ﾿ Forms of organic waste ﾿     5 

2.3.1 ﾿ Wet organic waste material ﾿     6

2.3.2     Dry organic waste material  ﾿     6

2.4 ﾿ Categories of organic waste ﾿     6

2.4.1 ﾿ Urban generated wastes ﾿     6

2.4.2 ﾿ Animal wastes ﾿     7

2.5 ﾿ Abattoir  ﾿     8

2.5.1    Activities that occur in abattoirs ﾿     9

2.5.2   Abattoir waste  ﾿   10 

2.6 ﾿ Saw dust ﾿   10

2.6.1     Practical uses ﾿   11 

CHAPTER THREE

3.0 ﾿ Materials and Methods ﾿   13

3.1    Study area  ﾿   13

3.2    Collection of samples ﾿   13

3.3    Media used and preparation ﾿   14

3.4    Enumeration of total heterotrophic bacteria from saw dust ﾿   14

3.5    Enumeration of total heterotrophic bacteria from abattoir waste water ﾿   15

3.6    Enumeration of cellulolytic bacteria from saw dust ﾿   15

3.7    Enumeration of cellulolytic bacteria from abattoir waste water ﾿   16

3.8    Identification and characterization of bacteria isolates ﾿   16

3.8.1  Microscopic examination ﾿   17

3.8.2  Gram staining  ﾿   17

3.8.3  Biochemical reaction tests ﾿   17

CHAPTER FOUR

4.0 ﾿ Results ﾿   20

CHAPTER FIVE 

5. 0   Discussion and Conclusion ﾿   26  

5.1    Discussion  ﾿   26

5.2    Conclusion ﾿   28

References