Investigation Of Alloxan Induced Diabetic Rats Exposed To Mosquito Insecticide:- Uka Nnenna K

ABSTRACT ix Malaria is a public health problem in many countries of the world especially in tropical and subtropical regions. In Nigeria, malaria is highly endemic accounting for 60% of outpatient visit. Mosquito insecticide is a vector control option used to prevent malaria in low income countries. The indiscriminate use of insecticides appears to increase the production of Iree radicals in organisms such as human and rats. The aim of this study was to investigate the effects of insecticide spray on oxidative and biochemical parameters of alloxan-induced diabetic rats exposed to mosquito insecticide. The animals in each group were anesthetized and blood samples were collected from the inferior vena cava after 21 days of exposure. A total of 20 wistar albino rats were used in this study and they were placed in four different groups, each group containing 5 rats. Group 1 was the control group, diabetes was induced in group 2 and 3 animals with alloxan, group 3 and group 4 animals were exposed to insecticide for 21 days. Plasma levels ofMDA, AST, Total hemoglobin, Glucose concentration was determined. Blood glucose concentration (mg/dl) of normal control rats, diabetic rats not exposed to insecticide, diabetic rats exposed to insecticide and non-diabetic rats exposed to insecticide at day 21 was 87.25±1.60 mg/dl, 338.75±26.80 mg/dl, 167.75±16.95mg/dl and 92.00±2.12 mg/dl respectively. A significant (p<0.05) increase in blood glucose level was observed at day 21 between control rats and diabetic rats exposed to insecticide fume. Results from this study showed that there was significant(p<0.05) increase in MDA levels between control group and diabetic and diabetic rats exposed to insecticide spray. Hemoglobin levels increased across the test group compared with the control group. There was a significant (p<0.05) increase in AST level of diabetic and exposed to insecticide compared to the control group. This study concludes that the oxidative stress was aggravated due to exposure ofanimals to the insecticide. This implies that activities of antioxidants were engulfed by free radicals.