Growing Pleurowtus Ostreatus Var. Florida Lawn Grass (Elusine Indica), Vertiver Grass (Vertivera Zizanoides), And Seam Weed (Chromolaena Odorata)

ABSTRACT

The comparison of Amount of mycelial development and fructification of Pleurotus ostreatus was carried out using 3 different agro wastes (lawn grass vertiver and seam weed) as substrate. Malt Extract Agar was used for the laboratory medium by mixing 10g of the Malt Extract Broth powder and agar powder in 500m1 of water. The preparation was autoclaved at 121 for 15 minutes and allowed to cool to 450 10mL of the medium was poured into sterile Petri dishes arid allowed to gel. Thoroughly washed cereal grain Millet (Penniseturn spp) was soaked overnight and then autoclaved. On cooling, pure culture of Pleurotus ostreatus were inoculated into the grains and incubated at 28 ± 1°C for about 2 weeks. Finally, dry vertivers grass, dry lawn grass and seam weed was soaked overnight and pasteurized for 2 hours at a temperature of 80°C in a gas heated drum. On cooling 40 g of the grain spawn were inoculated into the substrates fructification started 2 weeks after inoculation. Lawn grass produced more fruit bodies, followed by the vertiver and then the seam weed.