“Evaluation of The Hepato-Protective Activity of The Combination of Vitamin C And Vitamin E of Paracetamol Induced Toxicity In Wistar Albino Rats:- Adiole Confidence C.

ABSTRACT

Vitamins E and C are two of the chemical agents that have received wide attention due to reported Hepatoprotective effects. This could be due to the antioxidant activities of these two vitamins. Paracetamol as an analgesic can cause hepatotoxicity at high doses. This study is aimed at determining the efficacy ofthe combination ofVitamin C and Vitamin E in moping out the free radicals produced by paracetamol toxicity. Twenty-one male albino rats were used as my experimental model. The hepatoprotective effect of vitamins C and E against paracetamol-induced toxicity was assessed in these rats. Vitamin C at prophylactic dosage; (200mg, 150mg, lOOmg, 50mg, 25mg) and Vitamin E at prophylactic dosage; (500iu, 400iu, 300iu, 200iu, lOOiu) were separately administered orally to the test rats (groups 1 to 5) concomitantly with inducedparacetamol toxicity except group 7, which was the normal control. The experimental work lasted for seven days; after which the animals were sacrificed and blood samples were collected from each of them for the necessary liver function tests. After the tests, it was deduced paracetamol toxicity was observed to increase significantly (P < 0.05) activities of serum ALT, AST, and ALP in male albino rats. Oral administration of prophylactic doses of ascorbic acid and a-tocopherol decreased significantly (P < 0.05) activities ofthese parameters in male albino rats, compared with the nontreated test rats; but insignificant increase (P > 0.05), compared with the normal Based on the study, Co-administration of ascorbic acid and cion paracetamol-induced hepatotoxicity in wistar albino rats had significant effect in reducing damage incurred on liver during paracetamol toxicity;