Determination Of The Cyanogenic Potential Of Two New Cassava Cultivars And.The Hydrolytic Activites Of Their [3- Glucosidases:- Ekwelem, Esther A

ABSTRACT cultivars TME 419 and TMS 98/0505 was studied using spectrophotometric and enzymatic climatic environment. The cyanogenic potential of the root pulp in their unprocessed form cultivar and 0.21+0.05 mgCN'Kg'l for TMS 98/0.505 cultivar for garri and 36.06 for TME 419 and 34.16 for TMS 98/0505 respectively fetr the chips. The protein content of the xiv partially purified enzyme from these Cultivars gave 0.046mg/ml and 0.069mg/ml while mathematical analysis of the data from the degradation of Linamarin by the partially The cyanogenic potential of processed and unprocessed roots of two new cassava methods. Partial purification and some properties of the P-glucosidase from these cassava cultivars were also determined. These cultivars NRCRI, Umudikc but have not been assessed for cyanogenic potential under their new arc planted and distributed to farmers by ranged from 40.0 + 0.2 to 60.0 + 0.3 mgCN’Kg'1, while processing into garri and oven drying of the chips resulted in cyanogenic potential of 0.29+0.01 mgCN’Kg'1 for TME 419 purified enzyme generated HCN values that were used to construct Line Weaver Burk plot that gave apparent Km and Vmax yalue.s of 2L7mM and 5.0 x 10'2 pmol IICN/ml for TME 419 and 15.6mM and 4.9 x 10'2 pmol HCN/ml for TMS 98/0505 cultivars. The partially puiificd enzyme show.cd high degree of hydrolysis towards standard linamarin and cyanogenic glucosides ofthe new cassava cultivaers