Comparison Of Different Brands Of Toothpastes On Streptococcus Mutans.

ABSTRACT

Antimicrobial agents have been as chemotherapeutic agents to improve oral health. This in-vitro study was carried out to determine antimicrobial efficacy of different toothpastes against the oral pathogen (Streptococcus mutans). A total of four toothpastes(Sensodyne,Colgate,Close-up,Dabur-herbal) were tested for their antimicrobial activity against the oral pathogen, Streptococcus mutans. This was carried out by agar diffusion test and quantitative tube dilution test. Statistical analysis was performed using SPSS windows version 15 by applying the mean values using analysis of variance (ANOVA) with post-hoc least square differences (LSD) method (α = 0.05). The best toothpaste observed in this study was Sensodyne which had the highest zone of inhibition (18.33±2.52) during agar well diffusion test and was able to inhibit the test organism at the least concentration during quantitative tube dilution test. Results from the study showed that trichosan containing toothpaste formulations were more effective in controlling Streptococcus mutans compared to non-trichosan containing synthetic toothpaste.

TABLE OF CONTENT

Title Page ﾿ i

Dedication ﾿ ii

Acknowledgement ﾿ iii

Table of Contents ﾿ iv

List of Tables ﾿ v

Abstract ﾿ viii

CHAPTER ONE

1.0 ﾿ Introduction ﾿ 1

1.1 ﾿ Aims and objectives ﾿ 5 

CHAPTER TWO 

2.0 ﾿ Literature review ﾿ 6

2.1 ﾿ Toothpaste ﾿ 6 

2.2 ﾿ Dental caries ﾿ 22

2.3 ﾿ Mode of Streptococcus mutans transmission ﾿ 22

2.4 ﾿ Colonization of Streptococcus mutans ﾿ 23

CHAPTER THREE 

3.1 ﾿ Toothpaste sources ﾿ 25

3.2 ﾿ Collection of sample ﾿ 25

3.3 ﾿ Sterility of materials ﾿ 25

3.4 ﾿ Preparation of culture media ﾿ 25

3.4.1 ﾿ Preparation of the inoculums size ﾿ 26

3.5 ﾿ Identification and characterization of bacteria isolate ﾿ 26

3.6 ﾿ Gram staining ﾿ 26

3.7 ﾿ Biochemical test ﾿ 26

3.7.1 ﾿ Catalase ﾿ 27 

3.7.2 ﾿ Coagulase ﾿ 27

3.8 ﾿ Antimicrobial activity of formulated toothpaste ﾿ 27

3.9 ﾿ Preparation of dentifrice ﾿ 27

CHAPTER FOUR

 4.0 ﾿ Results ﾿ 28

CHAPTER FIVE 

5.1 ﾿ Conclusion ﾿ 31

5.2 ﾿ Recommendation ﾿ 31

References