Bacterial Contamination And Anaesthetic Efficacy Of Propofol Preserved Under Different Conditions ;- Anaba, Chidi E

ABSTRACT ix Propofol (2, 6 diisopropylphenol) is a phenolic compound unrelated to any other general anaesthetic which produces a short-term anaesthesia, followed by a rapid and smooth recovery.The drug is not water soluble, and is prepared as a milky white emulsion containing 10 mg Propofol, 100 mg soybean oil, 12 mg egg lecithin, and 22.5 mg glycerol per ml. It contains no preservative and the emulsion supports bacterial growth and endotoxin production. For this reason, it is recommended that once exposed to the air, the contents in the vial must be used within 8 hours or discarded thereafter. The aim of this study is to determine the effect of refrigeration and aseptic handling on bacterial contamination of Propofol and anaesthetic indices in Propofol anaesthetized Albino Wista rats. The study involes two sections, the first section is to determine time of bacterial contamination of preserved Propofol under different storage condition. This was done by placing 9 propofol vial in 3 groups (VR, VT, VS), Two hundred microlitres (0.2 milliliter) ofthe content of each vial in each group was aspirated for culture at 0 ,8 ,10, 11, 24, 48 hours, and 7days. Colony forming units were identified and counted. The contaminating organisms were identified. The second section was Sequel to the result of experiment 1, 9 vial of Propofol were (- \ a . Experiment 1 .Propofol 100 mg/g (NIRFOL® 1% Aculifc™, India), opened, grouped and preserved as those in , ,,was used fbr anaesthesia . A pool of 20 albino Wista rats was used fbr this experiment; The rats were randomly selected from the corresponding group, and anaesthetized by intraperitoneal injection. The indices of anaesthesia such as the duration of anaesthesia, palpebral reflex, corneal reflex, loss of abdominal pain, loss of tail pain sensation, Result; growth was observed fi*om VT {Bacillus subtilis, Streptococcus pneumonia) at 11 hours, while that of VR {klebsiella pneumonia, Bacillus subtilis, Staphylococcus epidermidis') and VS {Lactobacilli, Bacillus s s)growth was observed at 48 hours. In conclusion, the study showed that with proper aseptic handling and good storage facility, opened Propofol vial can stay up to 48hours without bacterial contamination.