Antimicrobial Effect Of Black Mustard Seed Extract On Some Pathogenic Bacteria

ABSTRACT

The oil of Brassica nigra commonly known as black mustard seed oil was extracted and evaluated for its antibacterial potential. The oil was extracted using aqueous and solvent extraction methods with water, ethanol and methanol. Black mustard seeds (Brassica nigra) oil extracts were found to have high antibacterial activity as was observed from the disc diffusion test done with the oil extracts; the oil extracts were infused into paper discs and planted on inoculated plates and incubated. The extracts were observed to have inhibitory effect on the pathogenic organisms (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes and Escherichia coli). The Minimum Inhibitory Concentration of the extracts were also determined. Phytochemicals such as tannins, saponins, flavonoids, alkaloids and phenols (1.13±0.01,1.01 ± 0.0.1,1.45 ± 0.00,1.37 ± 0.01, 1.08 ± 0.00) were also observed to be present in black mustard seed extracts.