ANTIBIOTIC SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL SPECIMENS

ABSTRACT

This study investigated the antibiotic susceptibility pattern of Pseudomonas aeruginosa isolated from clinical specimens, from in-patient, out-patient and intensive care unit of Abia State specialist hospital. Clinical specimens which include wound, urine, sputum, were analyzed for Pseudomonas aeruginosa. Forty eight isolates of Pseudomonas aeruginosa were isolated using  standard bacteriological procedure. The isolated pathogen showed resistance to Ciprofloxacin (33.33%), Streptomycin (83.33%), and showed susceptibility to Levoflaxocin (87.50%), Ceftriaxone (81.25%) Gentamycin (79.16%). From the result obtained, Pseudomonas aeruginosa isolates from in-patient and out patient, intensive care unit of Abia state specialist hospital were highly susceptible to Levofloxacin (87.50%) and Gentamycin (79.16%) than other antibiotics. Hence, the use of antibiotics should be monitored among the group to avoid further drug resistance.

TABLE OF CONTENT

Title page ﾿ i

Cover page ﾿ ii

Certification ﾿ iii

Dedication ﾿ iv

Acknowledgement ﾿ v

Abstract ﾿ vi

Table of content ﾿ vii

List of table ﾿ viii

CHAPTER ONE

 INTRODUCTION

1.0 ﾿ Background of the Study ﾿ 1

1.1 ﾿ Statement of Problem ﾿ 3 

1.2 ﾿ Significance of Study ﾿ 4 

1.3    ﾿ Limitations of the Study ﾿ 4 

CHAPTER TWO

2.1 ﾿ Literature Review ﾿ 7

2.2 ﾿ Clinical Manifestation ﾿ 8

2.3 ﾿ Antibiotic Resistance ﾿ 11

2.4 ﾿ Theoretical Framework ﾿ 13   

2.5 ﾿ Current Therapeutic Options ﾿ 14 

CHAPTER THREE 

METHODOLOGY

3.0 ﾿ Materials and Method ﾿ 20

3.1 ﾿ Setting ﾿ 20

3.2 ﾿ Specimen Collection and Processing ﾿ 20

3.3 ﾿ Media Preparation ﾿ 22

3.4 ﾿ Biochemical Test ﾿ 22

3.5 ﾿ Susceptibility Test ﾿ 24

CHAPTER FOUR

4.0 ﾿ Result ﾿ 25

4.1 ﾿ Patients and Specimen Data ﾿ 25

CHAPTER FIVE

5.1 ﾿ Discussion ﾿ 30

5.2 ﾿ Conclusion and Recommendation ﾿ 31

REFERENCES ﾿ 32

LIST OF TABLE

Table 4.1: Age and Sex Distribution of Patients Infected with 

Pseudomonas aeruginosa ﾿ 26

Table 4.2: Distribution of Specimens from where Pseudomonas 

aeruginosa was Isolated ﾿ 27

Table 4.3: Bacterial isolated from patients in various wards ﾿ 28

Table 4.4: Antibiotic Susceptibility Pattern of Pseudomonas

 aeruginosa from Clinical Specimens. ﾿ 29