Antibiotic Resistance Profile Of Escherichia Coli Isolated From Health Domestic Livestock In Umuwaoma Ngor Okpala L.G.A Imo State

ABSTRACT 

The antibiotic sensitivity pattern of Echerichia  coli isolated from faecal samples from healthy domestic livestock ( goats, local fowls and broilers)   was determined. Inoculation was carried out using the streaking method of inoculation and he sensitive was determined using the Disc diffusion method .50 E.coli strains were isolated from the three locations. In location 1 16(35.7%) were resistance to Ampicillin, 10(35.7%) to Gentamycin and 1(3.6%) to Ciprofloxacin. In location 2, 6(50.0%) were resistant to Ampicillin, 5(41.7%) to Gentamycin and streptomycin, while all the isolates were susceptible to Ciprofloxacin. In location 3, 6(60.0%) showed resistance to Ampicillin, while there is no evidence of resistant against Oxytetracyclin and ciprofloxacin 0(0.00%). Comparing Resistance rates according to all spruces of samples, 10(66.7%) of Goat isolates were resistance to Ampicillin, 7(46.7%) to Gentamycin and streptomycin respectively, 19(76.0%) from Local fowl isolates showed resistance to Ampicillin while the least percentage was recorded against ciprofloxacin 2(8.00%). From the broilers isolates 5(50.0%) were resistant to Ampicillin and Gentamycin and there was no evidence of resistance recorded against ciprofloxacin. The use of antibiotics in this feeding of these animals should be checked so as to reduce the development of drug resistance.

TABLE OF CONTENTS 

Title page ﾿ i

Certification ﾿             ii

Dedication ﾿ iii

Acknowledgment ﾿ iv

Table of content ﾿ v

List of Tables ﾿             vi 

Abstract ﾿ vii

CHAPTER ﾿ ONE

INTRODUCTION ﾿

1.1 ﾿   Introduction ﾿              1

1.2 ﾿   Aims and Objectives ﾿              2

CHAPTER TWO

 LITERATURE REVIEW ﾿ 3

2.1   Classification of Escherichia Coli ﾿             4

2.2  Morphology and Identification of Escherichia Coli ﾿                         4

2.3 classification of  Echerichia coli ﾿                         5 ﾿

2.4  Escherichia Coli Strains ﾿             5

2.5  Antigens ﾿             6

2.6  Epidemiology ﾿             6

2.7  Antibiotic Resistance of Escherichia Coli ﾿             7

CHAPTER THREE

MATERIALS AND METHODS

3.1 ﾿ Material Used ﾿            10

3.2 ﾿ Sample Collection ﾿            10

3.3 ﾿ Preparation of Media ﾿            10

3.4 ﾿ Sample Cultivation ﾿            11

3.5 ﾿ Characterization and Identification of Bacterial Isolates ﾿                       11

   3.5.2   Gram Staining ﾿            12

3.5.2   Indole Test ﾿            12

3.5.3   Catalase Test ﾿            12

3.5.4   Citrate Test ﾿            13

3.5.5   Oxidase Test ﾿            13

3.6     Antibiotic Susceptibility Test ﾿            14

CHAPTER FOUR

Results ﾿           15 

CHAPTER FIVE

 Discussion ﾿            20

5.1 Conclusion ﾿            21

 5.2 Recommendation                                                                                               21

    References