Amylase-Producing Abilities Of Bacillus Species And Aspergillus Species Isolated From Dumpsites In Umudike:- Chukwuka, Glory O

ABSTRACT

This study evaluated the potentials of Bacillus and Aspergi/lus to produce amylase enzyme. Soil sample were collected from different sites, diluted serially and plated on Tryptone Soya agar and Sabouraud Dextrose agar. The Bacillus Specie were identified using biochemical characterization protocol and screen for amylase production on nutrient agar amended with 1% starch. Among the Bacillus species isolated, four (4) isolates exhibited amylolytic power with hydrolysis halo diameter of between 12mm to 18 mm. Maximum amylase productions was observed for the Bacillus megaterium species with its diameter zone of clearance of 18.0mm on starch agar. In a similar way, one of the Aspergillus niger species isolated in this study produced clear haloes. The amylolytic ratio from this study showed that on three Bacillus isolates produced amylolytic ratio greater than 3 (good), and only one (1) showed less than 2 (poor). It could be concluded that this study has demonstrated the suitability of exploitation of the soil habitat for isolation of Bacteria and fungi capable of amylase production which can be used for several industrial applications