ABSTRACT
The isolation and identification of bacteria from waste dump soil was conducted by analysing samples of soil at different dumpsites along roads leading to different colleges and places within Michael Okpara University of Agriculture, Umudike. Bacterial analyses of soil samples using serial dilutions of the soil samples was carried out. Biochemical test was carried out to identify the particular types of bacteria isolated. The mean total bacteria count ranged from 3.4 x 105 (CFU)/g to 9.7 x 105(CFU)/g of soil. The bacteria isolated were Escherichia coli, Staphylococcus sp., Proteus sp., Salmonella sp., Shigellasp., Pseudomonas sp. and Klebsiellasp. The mean pH values ranged from 7.08 to 8.70, the mean moisture contents ranged from 8.2% to 17.5%, while the mean temperature values ranged 280C to 300C. The study revealed 7 genera of bacteria, 5 genera of which portend fecal contamination. It was inferred from this study that bacterial load poses a great risk not only to waste scavengers but also to the society thereby constituting some public health hazards. Aerobic bacteria are usually more likely contaminants of soil than anaerobic bacteria, this is especially considering the fact that the environment is aerobic. The study recommended that regular safe quality assessment of dump soil is essential considering the possible socio-economic and health implications of contaminated dumpsites.
TABLE OF CONTENTS
Title page i
Certification ii
Dedication iii
Acknowledgements iv
Table of Contents v
List of Tables viii
Abstract ix
CHAPTER ONE – INTRODUCTION 1
1.1. Aim and Objectives 5
CHAPTER TWO - LITERATURE REVIEW 6
2.1. Soil Description and Classification 7
2.2. Waste Management 7
2.3. Waste Handling Practices 8
2.3.1. Curb-side Collection 8
2.3.2. Incineration 8
2.3.3. Landfill 8
2.4. Causes of Improper Waste Disposal 9
2.5. Panacea to Waste Management 9
CHAPTER THREE - MATERIALS AND METHODS 11
3.1. Study Area 11
3.2. Sample Collection 11
3.3. Isolation and Enumeration of Bacterial Isolates 11
3.4. Characterization and Identification of Bacterial Isolates 12
3.4.1. Gram staining and Microscopy 12
3.5. Biochemical Test 13
3.5.1. Indole Test 13
3.5.2. Catalase Test 13
3.5.3. Citrate Utilization Test 13
3.5.4. Coagulase Test 14
3.5.5. Oxidase Test 14
3.6. Physicochemical Analysis 14
3.6.1. Determination of pH 14
3.6.2. Additional Tests 15
CHAPTER FOUR - RESULTS 16
CHAPTER FIVE: DISCUSSION, CONCLUSION AND RECOMMENDATION
5.1. Discussion 20
5.2. Conclusion 21
5.3. Recommendation 21
REFERENCES 23
EMMANUEL, M (2020). Isolation And Identification Of Bacteria From Selected Dumpsoil Within Michael Okpara Universityof Agriculture, Umudike. Mouau.afribary.org: Retrieved Nov 30, 2024, from https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-bacteria-from-selected-dumpsoil-within-michael-okpara-universityof-agriculture-umudike
MOUAU/12/23042, EMMANUEL. "Isolation And Identification Of Bacteria From Selected Dumpsoil Within Michael Okpara Universityof Agriculture, Umudike" Mouau.afribary.org. Mouau.afribary.org, 03 Jun. 2020, https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-bacteria-from-selected-dumpsoil-within-michael-okpara-universityof-agriculture-umudike. Accessed 30 Nov. 2024.
MOUAU/12/23042, EMMANUEL. "Isolation And Identification Of Bacteria From Selected Dumpsoil Within Michael Okpara Universityof Agriculture, Umudike". Mouau.afribary.org, Mouau.afribary.org, 03 Jun. 2020. Web. 30 Nov. 2024. < https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-bacteria-from-selected-dumpsoil-within-michael-okpara-universityof-agriculture-umudike >.
MOUAU/12/23042, EMMANUEL. "Isolation And Identification Of Bacteria From Selected Dumpsoil Within Michael Okpara Universityof Agriculture, Umudike" Mouau.afribary.org (2020). Accessed 30 Nov. 2024. https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-bacteria-from-selected-dumpsoil-within-michael-okpara-universityof-agriculture-umudike