Determination Of The Cyanogenic Potential Of Two New Cassava Cultivars And The Hydrolytic Activities Of Their B– Glucosidases
ABSTRACT
Fetch cyanoe,enic potential of
processed and unprocessed roots of two new cassava cultivars TME 41 9 and TMS
98/0505 was studied using spectrophotometric and enzymatic iiietliods. Partial
purl licUlioli and some PrOPCr1ieS ol the 3-g1ucosidasc from these cassava cultivars
were also determined. These cultivars are planted and distributed to farmers by
N RCR I. Umudike but have not been assessed for cyanogenic potential under
their new climatic environment. The cyanogcnic potential of the root pulp in
their unprocessed form ranged from 40.0 ± 0.2 to 60.0 ± 0.3 mgCNKg, while
processing into garri and oven drying of the chips resulted in cyanogenic
potential of 0.29±0.01 mgCNKg' for TME 419 cultivar and 0.2 1±0.05 mgCNKg for
TMS 98/0505 cu1tivar for garri and 36.06 for TME 419 and 34.16 br TMS 98/0505
respectively for the chips. The protein content of the partially purified
enzyme from these cuitivars gave 0.046mg/mi and 0.069mg/mi while mathematical
analysis of the data from the degradation of Linamarin by the partially purified
enzyme generated FICN values that were used to construct Line Weaver Burk plot that
gave apparent Km and Vmax values of 21.7mM and 5.0 x 10.2 imol FICN/ml for TME 419
and 15.6mM and 4.9 x 10.2 jimol I-ICN/ml for TMS 98/0505 cultivars. The
partially purified enzyme showed high degree of hydrolysis towards standard
linamarin and cyanogenic gi ucosides of the new cassava cultivars.
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EKWELEM, A (2021). Determination Of The Cyanogenic Potential Of Two New Cassava Cultivars And The Hydrolytic Activities Of Their B– Glucosidases . Mouau.afribary.org: Retrieved Dec 22, 2024, from https://repository.mouau.edu.ng/work/view/determination-of-the-cyanogenic-potential-of-two-new-cassava-cultivars-and-the-hydrolytic-activities-of-their-b-glucosidases-7-2
ADAKU, EKWELEM. "Determination Of The Cyanogenic Potential Of Two New Cassava Cultivars And The Hydrolytic Activities Of Their B– Glucosidases " Mouau.afribary.org. Mouau.afribary.org, 02 Jun. 2021, https://repository.mouau.edu.ng/work/view/determination-of-the-cyanogenic-potential-of-two-new-cassava-cultivars-and-the-hydrolytic-activities-of-their-b-glucosidases-7-2. Accessed 22 Dec. 2024.
ADAKU, EKWELEM. "Determination Of The Cyanogenic Potential Of Two New Cassava Cultivars And The Hydrolytic Activities Of Their B– Glucosidases ". Mouau.afribary.org, Mouau.afribary.org, 02 Jun. 2021. Web. 22 Dec. 2024. < https://repository.mouau.edu.ng/work/view/determination-of-the-cyanogenic-potential-of-two-new-cassava-cultivars-and-the-hydrolytic-activities-of-their-b-glucosidases-7-2 >.
ADAKU, EKWELEM. "Determination Of The Cyanogenic Potential Of Two New Cassava Cultivars And The Hydrolytic Activities Of Their B– Glucosidases " Mouau.afribary.org (2021). Accessed 22 Dec. 2024. https://repository.mouau.edu.ng/work/view/determination-of-the-cyanogenic-potential-of-two-new-cassava-cultivars-and-the-hydrolytic-activities-of-their-b-glucosidases-7-2