ABSTRACT
Antiseptics and Disinfectants are chemicals used extensively in hospitals, other health care centres and in homes to control the growth of microorganisms on living tissues and inanimate objects respectively. This study compared the efficacy of two disinfectants (HYPO and Ivy) and two antiseptics (Iodine and Carex sanitizer) against Staphylococcus aureus and Pseudomonas aeruginosa .The disk diffusion method and Rideal Walker Phenol coefficient test were used. For the antiseptics , Staphylococcus aureus was susceptible to Iodine(18) and was not susceptible to Carex sanitizer. For the test disinfectants, Staphylococcus aureus was less susceptible to Ivy (24) and more susceptible Hypo . For the antiseptics against Pseudomonas aeruginosa, it was more susceptible to Iodine and less susceptible to Carex sanitizer. The disinfectants against Pseudomonas aeruginosa, Pseudomonas aeruginosa was more susceptible to Hypo and was resistant to Ivy. The phenol coefficient of the test antiseptics against Staphylococcus aureus ranged from 5-6 . Carex sanitizer had the highest with 6. While Iodine had the least with 5.for the test disinfectants the phenol coefficient ranged from 6-7. Ivy had the least with 6 while Hypo had the highest with 7.For Pseudomonas aeruginosa , the phenol coefficient of the test antiseptics ranged from 5.3 to 6.3.Iodine had the least with 5.3 while Carex sanitizer had the highest with 6.3.For the test disinfectants, the phenol coefficient was 7.4 for both Hypo and Ivy. Thus for the phenol coefficient test, all the disinfectants and antiseptics showed strong bactericidal effect against the organisms. While for the disk diffusion, the result showed that Hypo had the strongest bactericidal effect while Carex sanitizer had the least bactericidal effect .The study concluded that Hypo should be used in our daily disinfection.
TABLE OF CONTENTS
Title Page i
Certification ii
Dedication iii
Acknowledgements iv
Table of Contents v
List of Tables vi
List of Figures vii
List of Plates viii
Abstract ix
CHAPTER ONE
1.0 Introduction 1
1.1 Aims and Objectives 4
CHAPTER TWO
2.0 Literature Review 5
2.1 History of Disinfectants 6
2.2 Disinfectants and antiseptics 6
2.3 Types of Disinfectants 7
2.4 Sources of contamination 13
2.5 Properties of A Disinfectant 13
2.6 General Features of Disinfectant 13
2,7 Factors influencing the efficacy of disinfectants and antiseptics 14
2.8 Neutralizers 15
2.9 General Features of the Test Organisms 15
2.10 Mechanism of Actions of Disinfectants against Bacteria 16
2.11 Resistant Action of Bacteria 16
2.12Pseudomonas sensitivity to antimicromial agents 17
2.13Staphylococcus sensitivity to antimicromial agents 17
2.14 Advantages and Disadvantages of Disinfectants 17
2.15 General Guidelines in the Use of Disinfectants 18
CHAPTER THREE
3.0 Material and Method 20
3.1 Collection of samples 20
3.2 Collection of test Organisms 20
3.3 Rideal Walker phenol coefficient test 20
3.4 Antimicrobial Susceptibility Testing 20
CHAPTER FOUR
4.0 RESULTS 22
CHAPTER FIVE
5.0 Discussion 39
5.1 Conclusion 42
5.2 Recommendations 41
References
EBUKA, M (2020). COMPARATIVE STUDY OF THE EFFICACY OF SOME DISINFECTANTS AND ANTISEPTICS ON Pseudomonas aeruginosa and Staphylococcus aureus. Mouau.afribary.org: Retrieved Nov 14, 2024, from https://repository.mouau.edu.ng/work/view/comparative-study-of-the-efficacy-of-some-disinfectants-and-antiseptics-on-pseudomonas-aeruginosa-and-staphylococcus-aureus
MOUAU/11/20024, EBUKA. "COMPARATIVE STUDY OF THE EFFICACY OF SOME DISINFECTANTS AND ANTISEPTICS ON Pseudomonas aeruginosa and Staphylococcus aureus" Mouau.afribary.org. Mouau.afribary.org, 04 May. 2020, https://repository.mouau.edu.ng/work/view/comparative-study-of-the-efficacy-of-some-disinfectants-and-antiseptics-on-pseudomonas-aeruginosa-and-staphylococcus-aureus. Accessed 14 Nov. 2024.
MOUAU/11/20024, EBUKA. "COMPARATIVE STUDY OF THE EFFICACY OF SOME DISINFECTANTS AND ANTISEPTICS ON Pseudomonas aeruginosa and Staphylococcus aureus". Mouau.afribary.org, Mouau.afribary.org, 04 May. 2020. Web. 14 Nov. 2024. < https://repository.mouau.edu.ng/work/view/comparative-study-of-the-efficacy-of-some-disinfectants-and-antiseptics-on-pseudomonas-aeruginosa-and-staphylococcus-aureus >.
MOUAU/11/20024, EBUKA. "COMPARATIVE STUDY OF THE EFFICACY OF SOME DISINFECTANTS AND ANTISEPTICS ON Pseudomonas aeruginosa and Staphylococcus aureus" Mouau.afribary.org (2020). Accessed 14 Nov. 2024. https://repository.mouau.edu.ng/work/view/comparative-study-of-the-efficacy-of-some-disinfectants-and-antiseptics-on-pseudomonas-aeruginosa-and-staphylococcus-aureus