ABSTRACT
Oil pollution is a worldwide threat to
the environment and the remediation of oil contaminated soils, sediments and
water is a major challenge for environmental research. Bioremediation is a
useful method for soil remediation. The objective of this study was to isolate
Actinomyces from the soil using membrane filter techniques and at the same time
use the isolates for remediation. Results showed polluted soil contained
average of 46.51% of total petroleum hydrocarbon (TPH) prior to remediation.
Two Actinomyces isolates were obtained, characterized (phenotypically and
genotypically) and identified as Streptomyces
carpaticus and Streptomyces xiamenensis respectively and subsequently used
for bioremediation trials. At an inoculation size of 1.0x108 cfu/ml
and a trial period of 90 days. The total petroleum hydrocarbon concentration in
the soil was reduced by 75.51% for Streptomyces
carpaticus and by 75.96% for Streptomyces
xiamenesis. Slight variations were recorded in the rate of degradation of
TPH by the two isolates. The rate of remediation was in the range of 1.066% per
day to 0.396% per day in treatment 1 (S.
carpaticus) and 0.926% per day to 0.386% per day in treatment 2 (S. xiamenensis). Remediation rates decreased
with time in both treatments. The efficiency of bioremediation process in
treated soils increased with time from 31.74% (day 15) to 65.61% (day 90) in
treatment 1 and from 27.04% (day 15) to 64.61% (day 90) in treatment 2. The
Total Heterotrophic Bacteria Count
(THBC) increased to maximum population of 6.13x108 cfu/g and 6.17x108
cfu/g in the soils respectively, there were however no significant reduction in
the total petroleum hydrocarbon of the control soil and that was attributed to
possible metabolism of the hydrocarbon by indigenous organism. The potentials
of the two Actinomyces isolates for
polluted soil was established by the finding of the work.
KINGSLEY, T (2024). Bioremediation Of Crude Oil Contaminated Soil Using Actinomyces Species In Onne, Rivers State:- Nlemadim, Kingsley T. Mouau.afribary.org: Retrieved Sep 08, 2024, from https://repository.mouau.edu.ng/work/view/bioremediation-of-crude-oil-contaminated-soil-using-actinomyces-species-in-onne-rivers-state-nlemadim-kingsley-t-7-2
TOCHI, KINGSLEY. "Bioremediation Of Crude Oil Contaminated Soil Using Actinomyces Species In Onne, Rivers State:- Nlemadim, Kingsley T" Mouau.afribary.org. Mouau.afribary.org, 25 Apr. 2024, https://repository.mouau.edu.ng/work/view/bioremediation-of-crude-oil-contaminated-soil-using-actinomyces-species-in-onne-rivers-state-nlemadim-kingsley-t-7-2. Accessed 08 Sep. 2024.
TOCHI, KINGSLEY. "Bioremediation Of Crude Oil Contaminated Soil Using Actinomyces Species In Onne, Rivers State:- Nlemadim, Kingsley T". Mouau.afribary.org, Mouau.afribary.org, 25 Apr. 2024. Web. 08 Sep. 2024. < https://repository.mouau.edu.ng/work/view/bioremediation-of-crude-oil-contaminated-soil-using-actinomyces-species-in-onne-rivers-state-nlemadim-kingsley-t-7-2 >.
TOCHI, KINGSLEY. "Bioremediation Of Crude Oil Contaminated Soil Using Actinomyces Species In Onne, Rivers State:- Nlemadim, Kingsley T" Mouau.afribary.org (2024). Accessed 08 Sep. 2024. https://repository.mouau.edu.ng/work/view/bioremediation-of-crude-oil-contaminated-soil-using-actinomyces-species-in-onne-rivers-state-nlemadim-kingsley-t-7-2