TABLE OF CONTENTS
Cover page i
Title page ii
Certificate Page iii
Dedication iv
Acknowledgement v
Contents vi
List of Tables vii
Abstract viii
CHAPTER ONE
1.0 INTRODUCTION 1
1.1 Justification of study 3
1.2 Specific objectives 3
1.3 General limitations in studying bacterial diversity 4
CHAPTER TWO
2.0 LITERATURE REVIEW 5
2.1 Introduction 5
2.1.1 Biogeochemical processes 8
2.1.2 What we don’t know 10
2.1.3 Increasing cultivation efficiency 11
2.2 Spatial heterogeneity 11
2.3 Inability to culture soil bacteria 13
2.4 Limitations of molecular-based methods 14
2.5 Taxonomic ambiguity of microbes 15
2.5.1 Methods of studying bacterial diversity in soil 17
2.6 Biochemical-based techniques to study bacterial diversity 18
2.7 Future perspective 19
CHAPTER THREE
3.0 MATERIALS AND METHODS 23
3.1 Sample collection 24
3.1.1 Determination of soil temperature 24
3.1.2 Determination of soil pH 24
3.1.3 Sampling depth 25
3.2 Processing of sample and media preparation 25
3.3 Isolation of bacteria from three different soil sample 28
3.4 Determination of groups of isolates based on differential techniques 29
3.5 Determination of groups of isolates based on structural staining 29
3.6 Biochemical tests 30
3.6.1 Indole test 30
3.6.2 Catalase test 30
3.6.3 Citrate utilization test 30
3.6.4 Coagulase test 30
3.6.5 Motility test 31
3.6.6 Urease test 31
3.6.7 Oxidase test 31
CHAPTER FOUR
4.0 RESULTS 32
CHAPTER FIVE
5.1 Discussion 34
5.2 Conclusion
References
LIST OF TABLES
Table Title Page
4.1 Bacterial isolates 32
4.2 Colony count 32
4.3 Morphology and Biochemical test 33
OZOR, O (2020). Study Of Soil Bacterial Diversity Based On Differential And Structural Staining. Mouau.afribary.org: Retrieved Nov 13, 2024, from https://repository.mouau.edu.ng/work/view/study-of-soil-bacterial-diversity-based-on-differential-and-structural-staining
OZOR, OZOR. "Study Of Soil Bacterial Diversity Based On Differential And Structural Staining" Mouau.afribary.org. Mouau.afribary.org, 03 Jun. 2020, https://repository.mouau.edu.ng/work/view/study-of-soil-bacterial-diversity-based-on-differential-and-structural-staining. Accessed 13 Nov. 2024.
OZOR, OZOR. "Study Of Soil Bacterial Diversity Based On Differential And Structural Staining". Mouau.afribary.org, Mouau.afribary.org, 03 Jun. 2020. Web. 13 Nov. 2024. < https://repository.mouau.edu.ng/work/view/study-of-soil-bacterial-diversity-based-on-differential-and-structural-staining >.
OZOR, OZOR. "Study Of Soil Bacterial Diversity Based On Differential And Structural Staining" Mouau.afribary.org (2020). Accessed 13 Nov. 2024. https://repository.mouau.edu.ng/work/view/study-of-soil-bacterial-diversity-based-on-differential-and-structural-staining