Starter Culture Fermentation Of African Oil Bean Seed (Pentaclethra Macrophylla Benth) With Bacillus Subtilis, Using Lactobacillus Fermentum As An Adjunct
ABSTRACT
Utilization of probiotic flora as starter
culture for controlled fermentation of African oil Bean (Pentaclethra Macrophylla
Ben/h) for ugba was evaluated. Isolated Bacillus substilis and Lactobacillus
fermentum from locally fermented ugba was used as starter cultures, singly and
in combination for the laboratory production of "ugba" for a period
of 48h at 35C. During the fermentation period, changes in proximate
composition, temperature, pH, titratable acidity and microbial count were
determined over 72 h periods. The use of starter culture of B. subtilis and L.
fermentum in singles and in combination produced ugba. The moisture, protein,
crude ash content of all samples were found increase throughout the
fermentation period, while the crude fibre and total solid decreased throughout
the fermentation period. However, the pH and titratable acidity of all samples
also decreased throughout the fermentation time (0-72h). The temperature and
microbial count (bacteria and fungi) increased within the first 24-48h and
decreased at the end of 72h. The ugba samples inoculated with B. subtilis and
L. fermentum had the highest percentage increase in moisture, protein.,
titratable acidity as fermentation time increased from 24-72h. They ranged
22.86-26.75%, 20.52-22.87% and 22.07-2.27% respectively. The use of B. sub/ills
as starter culture shortened the fermentation time of ugba from 3 to 2 days
while the use of L. fermentum singly, did not reduce the fermentation time of
ugba. Laclobacillusfermentum was used in single and as an adjunct for the
purpose of conveying health benefits when consumed. Ugba production appears to
be as a result of synergeric activity of fermenting microorganisms.
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ASIEGBU, M (2021). Starter Culture Fermentation Of African Oil Bean Seed (Pentaclethra Macrophylla Benth) With Bacillus Subtilis, Using Lactobacillus Fermentum As An Adjunct . Mouau.afribary.org: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/starter-culture-fermentation-of-african-oil-bean-seed-pentaclethra-macrophylla-benth-with-bacillus-subtilis-using-lactobacillus-fermentum-as-an-adjunct-7-2
MARK, ASIEGBU. "Starter Culture Fermentation Of African Oil Bean Seed (Pentaclethra Macrophylla Benth) With Bacillus Subtilis, Using Lactobacillus Fermentum As An Adjunct " Mouau.afribary.org. Mouau.afribary.org, 02 Aug. 2021, https://repository.mouau.edu.ng/work/view/starter-culture-fermentation-of-african-oil-bean-seed-pentaclethra-macrophylla-benth-with-bacillus-subtilis-using-lactobacillus-fermentum-as-an-adjunct-7-2. Accessed 24 Nov. 2024.
MARK, ASIEGBU. "Starter Culture Fermentation Of African Oil Bean Seed (Pentaclethra Macrophylla Benth) With Bacillus Subtilis, Using Lactobacillus Fermentum As An Adjunct ". Mouau.afribary.org, Mouau.afribary.org, 02 Aug. 2021. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/starter-culture-fermentation-of-african-oil-bean-seed-pentaclethra-macrophylla-benth-with-bacillus-subtilis-using-lactobacillus-fermentum-as-an-adjunct-7-2 >.
MARK, ASIEGBU. "Starter Culture Fermentation Of African Oil Bean Seed (Pentaclethra Macrophylla Benth) With Bacillus Subtilis, Using Lactobacillus Fermentum As An Adjunct " Mouau.afribary.org (2021). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/starter-culture-fermentation-of-african-oil-bean-seed-pentaclethra-macrophylla-benth-with-bacillus-subtilis-using-lactobacillus-fermentum-as-an-adjunct-7-2