ABSTRACT

 This study was aimed at elevating the seroprevalence and isolation of Helicobacter pylon among patient suspected of having stomach ulcer. Blood and stool samples were collected from patients living in different geographical distribution both male and female, young and old were examined. Out of 90 patients, 45 male and 45 female were selected between different age group ranging from 0-80&above. Two methods were used for laboratory investigation, which are stripe test known to be the sero test and the stool sample for isolation and identification of Helicobacter pylon. The stool sample were cultured and bacteria colonies were examined on the basis of Gram staining, colony morphology, biochemical reaction such as catalase, urease, oxidase and Hydrogen sulphide. From the findings, it was found that out of the 90 samples, 23.33% were seropositive and 62.22% were isolation positive. Almost 75% of patients were both seropositive and isolation positive, the highest percent of seropositive was 0-9 years, this was followed by both those within the age of 10-19 and 80&above(4.44%). there was no seropositive from 20- 39years but there were more seropositivity among female (14.44%). The conditions of Helicobacrer pylon are more seropositive among patient living in urban regions. The rate of infection in isolation was found to be more in age group 45-60 and less in age group below 15,Among the 75% of Helicobacterpylori infected patient, 72% are affected with ulcer and 20% found to have stomach ache. This study thus suggests that the prevalence of Helicobacter pylon infection in children below 0-9years is high in umuahia which could be due to lack of proper hygiene and improper sanitary facilities. Therefore proper hygiene and proper sanitary facilities should be taken.