Screening, Isolation And Characterization Of Antibiotic Producing Microorganisms From The Soil Samples In Michael Okpara University Of Agriculture, Umudike, Abia State .........
ABSTRACT
The soil environment constitutes
largely an untapped source of microorganisms with the potentiality of producing
antibiotics. The soil microorganism are responsible for a great percentage of
discovered bioactive secondary metabolites notably antibiotics. In this study,
10 •soil samples from different ecological sites were obtained and cultured in
microbiological media to isolate antibiotic producing microorganisms. Ten fold
serial dilution of each soil sample was carried out. 0. [ml of the serially
diluted samples were plated on Nutrient agar and Sabouraud Dextrose agar (SDA)
using the spread plate inoculation technique. A total of 25 isolates mostly
Gram- Positive organisms (Bacillus sp, Micrococcus sp and Actinomycetes were
recovered from the 10 samples analyzed. Of this number, 5 isolates was tested
against four organisms (Escherichia coil ATCC 25922, Staphylococcus aureus ATCC
25923, Pseudomonas aeruginosa ATCC 27153 , Mycohacierium sn?egnlatisATCC 14468)
for antibacterial activity. Out of the five isolates, only two (BL6I and BL42)
exhibited inhibitory effect against one of the test organisms (Pseudornonas
aeruginosa) with zones of inhibition of 9mm and 13mm respectively. The
remaining isolates showed no antimicrobial activity against the test organisms.
Considering the fact that soil harbours a wide range of microflora, there is
the tendency of finding out new species and strains that have antimicrobial
property. Hence this area of study needs an extensive and wide research on the
soil micro environment to discover potential antibiotics that can be effective against
microorganisms in the face of ft' nutrient antibiotic resistance.
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IBEH, H (2021). Screening, Isolation And Characterization Of Antibiotic Producing Microorganisms From The Soil Samples In Michael Okpara University Of Agriculture, Umudike, Abia State .......... Mouau.afribary.org: Retrieved Nov 16, 2024, from https://repository.mouau.edu.ng/work/view/screening-isolation-and-characterization-of-antibiotic-producing-microorganisms-from-the-soil-samples-in-michael-okpara-university-of-agriculture-umudike-abia-state-7-2
HENRIETTA, IBEH. "Screening, Isolation And Characterization Of Antibiotic Producing Microorganisms From The Soil Samples In Michael Okpara University Of Agriculture, Umudike, Abia State ........." Mouau.afribary.org. Mouau.afribary.org, 02 Aug. 2021, https://repository.mouau.edu.ng/work/view/screening-isolation-and-characterization-of-antibiotic-producing-microorganisms-from-the-soil-samples-in-michael-okpara-university-of-agriculture-umudike-abia-state-7-2. Accessed 16 Nov. 2024.
HENRIETTA, IBEH. "Screening, Isolation And Characterization Of Antibiotic Producing Microorganisms From The Soil Samples In Michael Okpara University Of Agriculture, Umudike, Abia State .........". Mouau.afribary.org, Mouau.afribary.org, 02 Aug. 2021. Web. 16 Nov. 2024. < https://repository.mouau.edu.ng/work/view/screening-isolation-and-characterization-of-antibiotic-producing-microorganisms-from-the-soil-samples-in-michael-okpara-university-of-agriculture-umudike-abia-state-7-2 >.
HENRIETTA, IBEH. "Screening, Isolation And Characterization Of Antibiotic Producing Microorganisms From The Soil Samples In Michael Okpara University Of Agriculture, Umudike, Abia State ........." Mouau.afribary.org (2021). Accessed 16 Nov. 2024. https://repository.mouau.edu.ng/work/view/screening-isolation-and-characterization-of-antibiotic-producing-microorganisms-from-the-soil-samples-in-michael-okpara-university-of-agriculture-umudike-abia-state-7-2