Screening, Isolation, And Characterization Of Antibiotic Producing Microorganisms From Soil Samples In Michael Okpara University Of Agriculture, Umudike Abia State.
ABSTRACT
This study was carried out to find the antimicrobial effects of soil microorganisms that have the
potential to produce antibiotics. Ten soil samples were collected from four different
Rhizosphere, Waterlog, Back of COLNAS, Forest soil) sites of Umudike. The isolated strains
were obtained by ten-fold serial dilution method plated on Sabrouraud Dextrose agar and
Nutrient agar by spread plate method. These were then assessed for antibacterial activity against
four pathogenic bacteria. Twenty six isolates were recovered and four showed evidence of
antibiotic. However, result indicated that three out of the four that showed evidence of antibiotic
were active against at least one of the test organisms, Escherichia co/i (ATCC 25922),
Staphyloccus aueus (ATCC 25923), Pseudomonas aeruginosa (ATCC27853), Mycobacteriurn
smegmatis (ATCC 28735). SIA2 and S3A19 exhibited high antibacterial activity against
Staphylococcus aureus, S4A24 showed slight zone of inhibition against Pseudomonas
aeruginosa, while S2A15 shown no clear zone of inhibition against test organisms. Therefore, it
is confirmed that soil samples screened for microorganisms producing antibiotic collected within
the University premises of Umudike were found to be potential source of novel antibiotics.
0.0
MICHAEL, U (2021). Screening, Isolation, And Characterization Of Antibiotic Producing Microorganisms From Soil Samples In Michael Okpara University Of Agriculture, Umudike Abia State. . Mouau.afribary.org: Retrieved Nov 16, 2024, from https://repository.mouau.edu.ng/work/view/screening-isolation-and-characterization-of-antibiotic-producing-microorganisms-from-soil-samples-in-michael-okpara-university-of-agriculture-umudike-abia-state-7-2
UNIVERSITY, MICHAEL. "Screening, Isolation, And Characterization Of Antibiotic Producing Microorganisms From Soil Samples In Michael Okpara University Of Agriculture, Umudike Abia State. " Mouau.afribary.org. Mouau.afribary.org, 02 Aug. 2021, https://repository.mouau.edu.ng/work/view/screening-isolation-and-characterization-of-antibiotic-producing-microorganisms-from-soil-samples-in-michael-okpara-university-of-agriculture-umudike-abia-state-7-2. Accessed 16 Nov. 2024.
UNIVERSITY, MICHAEL. "Screening, Isolation, And Characterization Of Antibiotic Producing Microorganisms From Soil Samples In Michael Okpara University Of Agriculture, Umudike Abia State. ". Mouau.afribary.org, Mouau.afribary.org, 02 Aug. 2021. Web. 16 Nov. 2024. < https://repository.mouau.edu.ng/work/view/screening-isolation-and-characterization-of-antibiotic-producing-microorganisms-from-soil-samples-in-michael-okpara-university-of-agriculture-umudike-abia-state-7-2 >.
UNIVERSITY, MICHAEL. "Screening, Isolation, And Characterization Of Antibiotic Producing Microorganisms From Soil Samples In Michael Okpara University Of Agriculture, Umudike Abia State. " Mouau.afribary.org (2021). Accessed 16 Nov. 2024. https://repository.mouau.edu.ng/work/view/screening-isolation-and-characterization-of-antibiotic-producing-microorganisms-from-soil-samples-in-michael-okpara-university-of-agriculture-umudike-abia-state-7-2