Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia

OGECHUKWU JOY MOUAU/12/23223 | 37 pages (6263 words) | Projects
Microbiology | Co Authors: OKOLI

ABSTRACT

The prevalence of Methicillin Resistant Staphylococcus aureus(MRSA) was assessed among pupils of Compassion Home Nursery/Primary School AhiaekeNdume, Umuahia. Nasal swabs were collected from 35 males and 15 females. The swabs were cultured on Manitol Salt Agar (MSA). After isolation, 45 samples yielded bacteria growth and 39 of the isolates were identified as Staphylococcus aureus. The antibiotic sensitivity of the isolate was done using Kirby bauer disc of diffusion with Abek multi disc antibiotic susceptibility containing 8 antibiotic disc. 32 (82.06%) of the isolates were resistant to Cloxacillin which is a methicillin antibiotics. The isolates were mostly sensitive to Gentamycin and Streptomycin with 35 (87.74%) and 39 (94.87%) sensitive to Gentamycin and Streptomycin respectively. The prevalence of Methicillin resistance among the isolates was 32 (82.06%) this is very high level of Methicillin resistance to Staphylococcus aureusin the environment and suggests the need for constant monitoring to avoid possible future epidemic of MRSA.


TABLE OF CONTENTS

Title page                                                                                                     i

Certification                                                                                                  ii

Dedication                                                                                                 iii

Acknowledgement                                                                                     iv

Table of contents                                                                                           v

List of tables                                                                                                 vi

Abstract                                                                                                       vii

CHAPTER ONE

1.1 INTRODUCTION          1

1.2 Aim of study                                                               3

1.3 Objectives of study                                                           3

CHAPTER TWO

2.0 LITERATURE REVIEW         4

2.1 General concept         4

2.2 Methicilin resistant Staphylococcus aureus          5

2.3 Antimicrobial chemotherapy         6

2.3.1 Antibiotic resistance and sensitivity                    8

2.4 Causes of antimicrobial resistance                                                       9

2.4.1 Treatment                                                                                                                             11

2.4.2Mechanism of action of methicillin                11

CHAPTER THREE

3.0 Materials and methods             12

3.1 Ethical consideration             12

3.2 Sample collection       12

3.3 Media preparation                                                                                         12

3.4Isolation of Staphylococcus aureus        12

3.5Confirmation test for Staphlococcusaureus        13

3.6 Standardization of test isolates                                                                             14

3.7 Antimicrobial susceptibility test                                                                 14

3.7.1 Examination and measurement of zone of inhibition                                 14

CHAPTER FOUR

4.0 RESULT        15

CHAPTER FIVE

5.1 DISCUSSION        19

5.2 Conclusion                                                                                                     19

5.3 Recommendation                                                                                       20

References                                                                                                         21

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APA

OGECHUKWU, M (2020). Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia. Mouau.afribary.org: Retrieved Nov 14, 2024, from https://repository.mouau.edu.ng/work/view/prevalence-of-nasal-carriage-of-methicilin-resistant-staphylococcus-aureus-among-pupils-of-compassion-home-nur-pri-school-ahiaeke-ndume-umuahia

MLA 8th

MOUAU/12/23223, OGECHUKWU. "Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia" Mouau.afribary.org. Mouau.afribary.org, 04 Jun. 2020, https://repository.mouau.edu.ng/work/view/prevalence-of-nasal-carriage-of-methicilin-resistant-staphylococcus-aureus-among-pupils-of-compassion-home-nur-pri-school-ahiaeke-ndume-umuahia. Accessed 14 Nov. 2024.

MLA7

MOUAU/12/23223, OGECHUKWU. "Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia". Mouau.afribary.org, Mouau.afribary.org, 04 Jun. 2020. Web. 14 Nov. 2024. < https://repository.mouau.edu.ng/work/view/prevalence-of-nasal-carriage-of-methicilin-resistant-staphylococcus-aureus-among-pupils-of-compassion-home-nur-pri-school-ahiaeke-ndume-umuahia >.

Chicago

MOUAU/12/23223, OGECHUKWU. "Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia" Mouau.afribary.org (2020). Accessed 14 Nov. 2024. https://repository.mouau.edu.ng/work/view/prevalence-of-nasal-carriage-of-methicilin-resistant-staphylococcus-aureus-among-pupils-of-compassion-home-nur-pri-school-ahiaeke-ndume-umuahia

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