ABSTRACT
The prevalence of Methicillin Resistant Staphylococcus aureus(MRSA) was assessed among pupils of Compassion Home Nursery/Primary School AhiaekeNdume, Umuahia. Nasal swabs were collected from 35 males and 15 females. The swabs were cultured on Manitol Salt Agar (MSA). After isolation, 45 samples yielded bacteria growth and 39 of the isolates were identified as Staphylococcus aureus. The antibiotic sensitivity of the isolate was done using Kirby bauer disc of diffusion with Abek multi disc antibiotic susceptibility containing 8 antibiotic disc. 32 (82.06%) of the isolates were resistant to Cloxacillin which is a methicillin antibiotics. The isolates were mostly sensitive to Gentamycin and Streptomycin with 35 (87.74%) and 39 (94.87%) sensitive to Gentamycin and Streptomycin respectively. The prevalence of Methicillin resistance among the isolates was 32 (82.06%) this is very high level of Methicillin resistance to Staphylococcus aureusin the environment and suggests the need for constant monitoring to avoid possible future epidemic of MRSA.
TABLE OF CONTENTS
Title page i
Certification ii
Dedication iii
Acknowledgement iv
Table of contents v
List of tables vi
Abstract vii
CHAPTER ONE
1.1 INTRODUCTION 1
1.2 Aim of study 3
1.3 Objectives of study 3
CHAPTER TWO
2.0 LITERATURE REVIEW 4
2.1 General concept 4
2.2 Methicilin resistant Staphylococcus aureus 5
2.3 Antimicrobial chemotherapy 6
2.3.1 Antibiotic resistance and sensitivity 8
2.4 Causes of antimicrobial resistance 9
2.4.1 Treatment 11
2.4.2Mechanism of action of methicillin 11
CHAPTER THREE
3.0 Materials and methods 12
3.1 Ethical consideration 12
3.2 Sample collection 12
3.3 Media preparation 12
3.4Isolation of Staphylococcus aureus 12
3.5Confirmation test for Staphlococcusaureus 13
3.6 Standardization of test isolates 14
3.7 Antimicrobial susceptibility test 14
3.7.1 Examination and measurement of zone of inhibition 14
CHAPTER FOUR
4.0 RESULT 15
CHAPTER FIVE
5.1 DISCUSSION 19
5.2 Conclusion 19
5.3 Recommendation 20
References 21
OGECHUKWU, M (2020). Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia. Mouau.afribary.org: Retrieved Nov 14, 2024, from https://repository.mouau.edu.ng/work/view/prevalence-of-nasal-carriage-of-methicilin-resistant-staphylococcus-aureus-among-pupils-of-compassion-home-nur-pri-school-ahiaeke-ndume-umuahia
MOUAU/12/23223, OGECHUKWU. "Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia" Mouau.afribary.org. Mouau.afribary.org, 04 Jun. 2020, https://repository.mouau.edu.ng/work/view/prevalence-of-nasal-carriage-of-methicilin-resistant-staphylococcus-aureus-among-pupils-of-compassion-home-nur-pri-school-ahiaeke-ndume-umuahia. Accessed 14 Nov. 2024.
MOUAU/12/23223, OGECHUKWU. "Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia". Mouau.afribary.org, Mouau.afribary.org, 04 Jun. 2020. Web. 14 Nov. 2024. < https://repository.mouau.edu.ng/work/view/prevalence-of-nasal-carriage-of-methicilin-resistant-staphylococcus-aureus-among-pupils-of-compassion-home-nur-pri-school-ahiaeke-ndume-umuahia >.
MOUAU/12/23223, OGECHUKWU. "Prevalence Of Nasal Carriage Of Methicilin Resistant Staphylococcus Aureus Among Pupils Of Compassion Home Nur/Pri School, Ahiaeke Ndume, Umuahia" Mouau.afribary.org (2020). Accessed 14 Nov. 2024. https://repository.mouau.edu.ng/work/view/prevalence-of-nasal-carriage-of-methicilin-resistant-staphylococcus-aureus-among-pupils-of-compassion-home-nur-pri-school-ahiaeke-ndume-umuahia