Phytochemical Analysis And Antibacterial Activity Of Ocimum Gratissimum (Scent Leaf) On Some Selected Gram Positive & Gram Negative Bacteria
ABSTRACT
Antibacterial properties of Ocimum gratissimum was determined and tested against pure cultures of clinical isolates of Escherichia coli and Staphylococcus aureus. Water and Ethanol were used for the extraction of the active constituent of the plants. The phytochemical analysis carried out on the plant revealed the presence of Tannin, Saponin, glycoside and alkaloid. Aqueous extract of Ociumum gratissimum showed no inhibitory effect on Escherichia coli. The extract inhibited Staphylococcus aureus only at 100% and 80% concentration with zone of inhibition diameter of 100mm and 9mm the Ethanol extract of the plant showed the mean zone diameter of inhibition. The highest zone of inhibition 30mm and 20mm was recorded against Escherichia coli at 100% and 80% concentration. The least inhibitory effect was observed with Staphylococcus aureus (7mm) at 20% concentration the minimum inhibitory concentration of the different plant extract showed that there was no activity observed with aqueous extract against Escherichia coli, but the minimum inhibitory concentration of the ethanolic extract against Staphylococcus aureus and Escherichia coli was 80%. The result of this study suggests the possibility of using the ethanolic extract in treating the diseases caused by the test organisms.
0.0
NWANKWO, C (2022). Phytochemical Analysis And Antibacterial Activity Of Ocimum Gratissimum (Scent Leaf) On Some Selected Gram Positive & Gram Negative Bacteria. Mouau.afribary.org: Retrieved Nov 15, 2024, from https://repository.mouau.edu.ng/work/view/phytochemical-analysis-and-antibacterial-activity-of-ocimum-gratissimum-scent-leaf-on-some-selected-gram-positive-gram-negative-bacteria-7-2
C, NWANKWO. "Phytochemical Analysis And Antibacterial Activity Of Ocimum Gratissimum (Scent Leaf) On Some Selected Gram Positive & Gram Negative Bacteria" Mouau.afribary.org. Mouau.afribary.org, 16 Feb. 2022, https://repository.mouau.edu.ng/work/view/phytochemical-analysis-and-antibacterial-activity-of-ocimum-gratissimum-scent-leaf-on-some-selected-gram-positive-gram-negative-bacteria-7-2. Accessed 15 Nov. 2024.
C, NWANKWO. "Phytochemical Analysis And Antibacterial Activity Of Ocimum Gratissimum (Scent Leaf) On Some Selected Gram Positive & Gram Negative Bacteria". Mouau.afribary.org, Mouau.afribary.org, 16 Feb. 2022. Web. 15 Nov. 2024. < https://repository.mouau.edu.ng/work/view/phytochemical-analysis-and-antibacterial-activity-of-ocimum-gratissimum-scent-leaf-on-some-selected-gram-positive-gram-negative-bacteria-7-2 >.
C, NWANKWO. "Phytochemical Analysis And Antibacterial Activity Of Ocimum Gratissimum (Scent Leaf) On Some Selected Gram Positive & Gram Negative Bacteria" Mouau.afribary.org (2022). Accessed 15 Nov. 2024. https://repository.mouau.edu.ng/work/view/phytochemical-analysis-and-antibacterial-activity-of-ocimum-gratissimum-scent-leaf-on-some-selected-gram-positive-gram-negative-bacteria-7-2