ASBTRACT

 This study was used to determine the microbial implication of hiobased fluid. Lard used in this was processed after been purchase from local sellers within Umuahia metropolis. After the samples were cultured on solid media using pour plate techniques. The bacteria species was identified as Staphylococcus aureus, Escherichia coli and Salmonella species while the fungi species include Asper gillus niger and Aspergillus.flavus. Count from the used were higher than those of the unused samples it was also observed that used sample obtained from Umuariga junction recorded the highest bacteria and fungal count at 2.26x10° (CFU/ml) and 2.31x10° and 1.67x10° CFU/ml for bacteria and fungal respectively. From the finding in this study, it was obtained that the Bacillus spp species and Escherichia coli was the most frequently occurring isolates with percentage occurrence of (26.12%), with 4spergillus niger was observed to be the most predominate and occurring fungi spp at (57.14%). Additive was added to stop rancidity, corrosion, foaming and improve oxidative stability. The help of this additive made this fluid better than those available in the market. Statistical analysis showed that the significant difference between mean count of the used and unused formulated oil sample at p<0.005