ABSTRACT
Toothbrushes are an environment for
transfer of microbes, their retention and growth. The aim of the study was to
identify the bacteria load of contaminated toothbrushes, isolate Streptococcus mutans from used manual
tooth brush and to access the microbial growth of Streptococcus mutans and Labctobacillus
in tooth brush. A total number of twenty (20) samples were collected within and
outside Michael Okpara University of Agriculture Umudike, Abia State. The
samples were obtained from used tooth brushes used by individuals that resides
within the confines of Umudike. The total bacteria count of each sample. The
total bacterial count of 20 samples ranges from 0.31 × 105 to 1.44 ×
105 cfu/ml. The morphological identification, biochemical
identification, Gram reaction and sugar fermentation of the bacteria isolates
from the swab samples obtained from the used toothbrushes were taken and they
are Pseudomonas aeruginosa, Escherichia
coli, Staphylococcus aureus, and Streptococcus mutans. Staphylococcus aureus has
the highest percentage of occurrence from the used toothbrush with an
occurrence of 32.5%, followed by Escherichia
coli with an occurrence of 27.5%, then Streptococcus
mutans with an occurrence of 22.5% and Pseudomonas
aeruginosa with the least occurrence of 17.5%. Toothbrushes become highly
contaminated after everyday use and can be carriers of microorganisms,
increasing the risk of diseases caused by oral biofilm in healthy people.
IBEAWUCHI, I (2025). Microbial Evaluation Of Manually Used Toothbrushes From Michael Okpara University Of Agriculture Students:- Ibeawuchi, Stephanie C. Mouau.afribary.org: Retrieved Apr 25, 2025, from https://repository.mouau.edu.ng/work/view/microbial-evaluation-of-manually-used-toothbrushes-from-michael-okpara-university-of-agriculture-students-ibeawuchi-stephanie-c-7-2
IBEAWUCHI, IBEAWUCHI. "Microbial Evaluation Of Manually Used Toothbrushes From Michael Okpara University Of Agriculture Students:- Ibeawuchi, Stephanie C" Mouau.afribary.org. Mouau.afribary.org, 11 Apr. 2025, https://repository.mouau.edu.ng/work/view/microbial-evaluation-of-manually-used-toothbrushes-from-michael-okpara-university-of-agriculture-students-ibeawuchi-stephanie-c-7-2. Accessed 25 Apr. 2025.
IBEAWUCHI, IBEAWUCHI. "Microbial Evaluation Of Manually Used Toothbrushes From Michael Okpara University Of Agriculture Students:- Ibeawuchi, Stephanie C". Mouau.afribary.org, Mouau.afribary.org, 11 Apr. 2025. Web. 25 Apr. 2025. < https://repository.mouau.edu.ng/work/view/microbial-evaluation-of-manually-used-toothbrushes-from-michael-okpara-university-of-agriculture-students-ibeawuchi-stephanie-c-7-2 >.
IBEAWUCHI, IBEAWUCHI. "Microbial Evaluation Of Manually Used Toothbrushes From Michael Okpara University Of Agriculture Students:- Ibeawuchi, Stephanie C" Mouau.afribary.org (2025). Accessed 25 Apr. 2025. https://repository.mouau.edu.ng/work/view/microbial-evaluation-of-manually-used-toothbrushes-from-michael-okpara-university-of-agriculture-students-ibeawuchi-stephanie-c-7-2