ABSTRACT

This study identifies the bacterial load of a produced unconventional fluid, to isolate fungi in the pressure transmitting fluid and to identify the best substance that can keep the fat in liquid form without microbial contamination. Fats used in this study were purchased from local sellers at Umuariaga junction. It was rendered using wet preparation. The mean microbial count for both used and unused sample shows that the total heterotrophic bacterial count of the used sample was 2.20 ×10⁵ cfu/ml. The total coliform plate count of the used sample was 2.26 ×10⁵ while the total fungal plate count was 2.31 ×10⁵. The total heterotrophic bacterial count of the unused sample was 1.91 ×10⁵ cfu/ml, the total coliform plate count of the unused sample was 1.20 ×10⁵ while the total fungal plate count was 1.67 ×10⁵. Bacillus species has the highest occurrence of 26.12% which is followed by Staphyloccus aureus which has an occurrence of 21.71%, Pseudomonas species has an occurrence of 21.74% then Escherichia coli with an occurrence of 17.4% while   Salmonella species has the least occurrence of 13.00%. Aspergillus flavus and Aspergillus niger 42.86% and 57.14% occurrence respectively. Additives such as Vitamin C and E were added to prevent rancidity and enhances its oxidative stability, Zigma Aldrich and Phosphoric acid acts as corrosive inhibitors, Kerosene reduces the gelling and viscous nature of pressure transmitting fluids. The pressure transmitting fluid was proven to be effective after it was being tested on a Toyota Camry vehicle. The statistical analysis shows that there was no significant difference between the used and unused samples.