ABSTRACT
A total of Ten (10) Samples were collected from barbing salons in Umuahia Metropolis with the aid of sterile swab stick and transported to laboratory for microbial analysis. The biochemical characterization of isolates revealed the genera of Pseudomonas sp, Bacillus sp, E.coli, Micrococcus luteus, Proteus sp and Serratia marcesens and the fungal characterization revealed the presence of Aspergillus niger, Rhodotorula sp, Fusarium oxyporum and Aspergillus flavus. The percentage occurrence of isolates from user hair brushes and clippers in barbing salons samples showed that Pseudomonas sp had the highest occurrence (25%) followed by E.coli 18.75%, Micrococcus luteus (18.75%) , Proteus sp (12.5%), while Serratia marcesens (6.25%) while Bacillus sp (6.25%) had the least occurrence. The percentage occurrence of fungal isolates from user hair brushes and clippers in barbing salons samples showed that Aspergillus niger (38.5%) had the highest percentage occurrence followed by Rhodotorula sp (30.8%) while Fusarium oxysporum (15.4%) and Aspergillus flavus (15.4%) showed the lowest occurrence from user hair brushes and clippers in barbing salons samples. Antibiotics sensitivity test of the isolated bacteria revealed that all the six recorded (100%) sensitivity on Ciprofloxacin and Gentamycin while Proteus sp, Pseudomonas aeruginosa and E.coli were all resistant to Levofloxacin and Rifampicin respectively.
TABLE OF CONTENTS
Title Page i
Certification ii
Dedication iii
Acknowledgements iv
Table of Contents v
Lists of Tables vii
Abstract viii
CHAPTER ONE
1.0 Introduction 1
1.1 Aim and Objectives 3
CHAPTER TWO
2.0 Literature Review 4
2.1 Folliculitis 9
2.2 Tinea 10
CHAPTER THREE
3.0 Materials and Methods 14
3.1 Sample Collection 14
3.2 Media Used 14
3.3 Sterilization 15
3.5 Determination of Bacterial Isolation 15
3.5.1 Grams Staining 16
3.6 Biochemical Characteristics of The Isolates 16
3.6.2 Indole Test 17
3.6.3 Citrate Utilization 17
3.6.4 Sugar Fermentation 17
3.6.5 Oxidase Test 18
3.6.6 Coagulase Test 18
3.6.7 Urease Test 18
3.6.8 Methyl Red –Voges Proskauer (MRVP) Test 19
3.7 Characterization And Identification of The Fungal isolates 19
3.7.1 Lactophenol Cotton Blue Staining 20
3.8 Antibiotic Susceptibility Testing 20
CHAPTER FOUR
4.0 Results 21
CHAPTER FIVE
5.0 Discussion, Conclusion and Recommendation 30
5.1 Discussion 30
5.2 Conclusion 32
5.3 Recommendation 32
References
AGHAELINA, A (2020). Microbial Contamination Of Multiple Users Of Hair Brushes And Clippers In Barbing Salons In Umuahia, Metropolis, Abia State. Mouau.afribary.org: Retrieved Nov 13, 2024, from https://repository.mouau.edu.ng/work/view/microbial-contamination-of-multiple-users-of-hair-brushes-and-clippers-in-barbing-salons-in-umuahia-metropolis-abia-state
AGHAELINA, AGHAELINA. "Microbial Contamination Of Multiple Users Of Hair Brushes And Clippers In Barbing Salons In Umuahia, Metropolis, Abia State" Mouau.afribary.org. Mouau.afribary.org, 03 Jun. 2020, https://repository.mouau.edu.ng/work/view/microbial-contamination-of-multiple-users-of-hair-brushes-and-clippers-in-barbing-salons-in-umuahia-metropolis-abia-state. Accessed 13 Nov. 2024.
AGHAELINA, AGHAELINA. "Microbial Contamination Of Multiple Users Of Hair Brushes And Clippers In Barbing Salons In Umuahia, Metropolis, Abia State". Mouau.afribary.org, Mouau.afribary.org, 03 Jun. 2020. Web. 13 Nov. 2024. < https://repository.mouau.edu.ng/work/view/microbial-contamination-of-multiple-users-of-hair-brushes-and-clippers-in-barbing-salons-in-umuahia-metropolis-abia-state >.
AGHAELINA, AGHAELINA. "Microbial Contamination Of Multiple Users Of Hair Brushes And Clippers In Barbing Salons In Umuahia, Metropolis, Abia State" Mouau.afribary.org (2020). Accessed 13 Nov. 2024. https://repository.mouau.edu.ng/work/view/microbial-contamination-of-multiple-users-of-hair-brushes-and-clippers-in-barbing-salons-in-umuahia-metropolis-abia-state