Isolation And Screening Of Exopolysaccharide Producing Lactic Acid Bacteria Isolated From Ugba (Pentaclethra Macrophyla)
ABSTRACT
Exopolysaccharides are high molecular weight polymers that are composed of sugar residues and are secreted by microorganisms into the surrounding. This work is aimed at isolating and screening of exopolysacchride producing lactic acid bacteria (LAB) isolated from Ugba (Pentaclethramacrophylla). Locally produced samples of Ugba were gotten from an open market in Ekwulobia, Anambra State, Nigeria. The samples were plated on M17 agar, MRS (de Man Rogosa and Sharp) agar and on Nutrient Agar. A total of 25 presumptive LAB isolates were gotten after some physiological, morphological and biochemical test were carried out. 8 isolates (EXO1, EXO2, EXO3, EXO4, EXO5, EXO6, EXO7, EXO8) showed exopolyaccharide activity which was confirmed by the determination of their ropy and mucoid characteristics which was done using different sugar supplements as carbon sources. The length of strand (ropiness) formed by ropy strains plated on M17, MRS and NA supplemented with sucrose, lactose and glucose as carbon sources were calculated and recorded, while (EXO1, EXO2, EXO3, EXO6, and EXO8) strains plated on MRS and M17 agar showed better mucoid activity. This shows that the length of the strands exhibited by the ropy and mucoid isolates of LAB varied according to strain and carbon source.
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NWAMARA, N (2022). Isolation And Screening Of Exopolysaccharide Producing Lactic Acid Bacteria Isolated From Ugba (Pentaclethra Macrophyla). Mouau.afribary.org: Retrieved Nov 15, 2024, from https://repository.mouau.edu.ng/work/view/isolation-and-screening-of-exopolysaccharide-producing-lactic-acid-bacteria-isolated-from-ugba-pentaclethra-macrophyla-7-2
N, NWAMARA. "Isolation And Screening Of Exopolysaccharide Producing Lactic Acid Bacteria Isolated From Ugba (Pentaclethra Macrophyla)" Mouau.afribary.org. Mouau.afribary.org, 21 Feb. 2022, https://repository.mouau.edu.ng/work/view/isolation-and-screening-of-exopolysaccharide-producing-lactic-acid-bacteria-isolated-from-ugba-pentaclethra-macrophyla-7-2. Accessed 15 Nov. 2024.
N, NWAMARA. "Isolation And Screening Of Exopolysaccharide Producing Lactic Acid Bacteria Isolated From Ugba (Pentaclethra Macrophyla)". Mouau.afribary.org, Mouau.afribary.org, 21 Feb. 2022. Web. 15 Nov. 2024. < https://repository.mouau.edu.ng/work/view/isolation-and-screening-of-exopolysaccharide-producing-lactic-acid-bacteria-isolated-from-ugba-pentaclethra-macrophyla-7-2 >.
N, NWAMARA. "Isolation And Screening Of Exopolysaccharide Producing Lactic Acid Bacteria Isolated From Ugba (Pentaclethra Macrophyla)" Mouau.afribary.org (2022). Accessed 15 Nov. 2024. https://repository.mouau.edu.ng/work/view/isolation-and-screening-of-exopolysaccharide-producing-lactic-acid-bacteria-isolated-from-ugba-pentaclethra-macrophyla-7-2