Isolation And Identification Of Microorganisms From The External Surface Of Housefly (Musca Domestica) In Umuahia Metropolis, Abia Sta Te
ABSTRACT
Housefly, M. domestica has long been
considered as disease vectors. This study was • focused on the isolation and
identification of pathogenic microorganisms from the external surface of
houseflies. The external surface of houseflies collected from five different
sites (market, clinic, refuse dump, 'restaurant and broken sewage) in Umuahia
metropolis were screened for microbial pathogens. The sample was prepared by
placing the flies in sterile universal containers containing normal saline and
shaken vigorously. Tbe isolates obtained from the samples inoculated on culture
plates were identified and characterized by their morphology, microscopic
appearance and biochemical reaction. The most frequent isolates were E. coli
(22.29%), Klebsiella spp ( 16.56%), S. aureus (14.64%), Aspergillus spp
(28.33%), Mucor spp (21.67%), E. histolytica (32.73%) and E. nana (30.91 %).
Quantitative analysis of the total viable count and mean load of the isolates
from all sites revealed higher values from the broken sewage and refuse
compared to other sites. Bacterial isolates from flies obtained from the clinic
exhibited greater percentage of resistant to commonly used antibiotics
(gentamicin, streptomycin, septrin, peflacine, ofloxacin, ceporex, ceftriaxone
and ampicillin. Thus, houseflies % can transmit microbial pathogens through
their external surface particularly drug resistant ones although good and
adequate sanitation is instrumental to the control of these flies
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CHINENYE, L (2022). Isolation And Identification Of Microorganisms From The External Surface Of Housefly (Musca Domestica) In Umuahia Metropolis, Abia Sta Te. Mouau.afribary.org: Retrieved Nov 14, 2024, from https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-microorganisms-from-the-external-surface-of-housefly-musca-domestica-in-umuahia-metropolis-abia-sta-te-7-2
LOVETH, CHINENYE. "Isolation And Identification Of Microorganisms From The External Surface Of Housefly (Musca Domestica) In Umuahia Metropolis, Abia Sta Te" Mouau.afribary.org. Mouau.afribary.org, 05 Dec. 2022, https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-microorganisms-from-the-external-surface-of-housefly-musca-domestica-in-umuahia-metropolis-abia-sta-te-7-2. Accessed 14 Nov. 2024.
LOVETH, CHINENYE. "Isolation And Identification Of Microorganisms From The External Surface Of Housefly (Musca Domestica) In Umuahia Metropolis, Abia Sta Te". Mouau.afribary.org, Mouau.afribary.org, 05 Dec. 2022. Web. 14 Nov. 2024. < https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-microorganisms-from-the-external-surface-of-housefly-musca-domestica-in-umuahia-metropolis-abia-sta-te-7-2 >.
LOVETH, CHINENYE. "Isolation And Identification Of Microorganisms From The External Surface Of Housefly (Musca Domestica) In Umuahia Metropolis, Abia Sta Te" Mouau.afribary.org (2022). Accessed 14 Nov. 2024. https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-microorganisms-from-the-external-surface-of-housefly-musca-domestica-in-umuahia-metropolis-abia-sta-te-7-2