ABTRACT
Isolation and identification of microorganism on various denominations of the Nigerian paper money (N5, N10, N20, N50, N100, N200, N500, N1000), the money sample were collected randomly from meat sellers in Ariaria international market Aba. These currency notes were examined for possible contamination by disease-causing bacteria and fungi and hence their presence on surface could serve as a source of cross-transmission of bacteria and fungi infection to marketers and the general community. Some of the microorganisms that can be found on naira notes isolated on different medium such as Mannitol salt agar, MacConkey agar, man Rogosa-Sharpe medium, Sabouraud dextrose agar, nutrient agar, salmonella-shigella agar Blood agar with their percentage occurrence are Escherichia coli (20.5%), Salmonella spp(12.8%), Shigella spp(14.2%), Staphylococcus aureus (20.5%), Pseudomonas aeruginosa(5.1%) and Bacillus spp(7.0). Fungi include: Aspergillus spp(4.5%) and Rhizopus spp(3.2%).Further, the mean count of the isolates was indicated with the total heterotrophic bacteria count(THBC) ranging from ₦100(2.9104cfu/g) to ₦20(1.3104cfu/g),coliform count ranging from ₦5(6.2103cfu/g) to ₦1000(3.1103 cfu/g),staphylococci count ₦100(6.5103cfu/g ) to count ₦500(3.2103cfu/g).lactobacillus count ranges from ₦200(3.2103cfu/g) to count ₦500(2.2103cfu/g), salmonella shigella count ranges from count ₦5(5.9103cfu/g) to count ₦20(3.1103cfu/g) and fungi count ranges from count ₦500(9.0102cfu/g) to count ₦1000(11102cfu/g),their presence on such surfaces could serve as a source of cross-transmission of bacteria and fungi. The bacteria isolates is to be subjected to a broad spectrum of erythromycin, augumentin, ceftazidine, cefuroxime, gentamycin, cefixime, oflaxacin, cloxacillin, ciprofloxacin, nitrofurantiol and ceftriaxone. However, using wallets to keep money and maintaining proper hygiene while handling money is recommended for the entire population.
TABLE OF CONTENTS
Title page i
Certification ii
Dedication iii
Acknowledgements iv
Table of Contents v
List of Tables viii
List of figures ix
Abstract x
CHAPTER ONE: Introduction 1
1.1 Objective of study 4
CHAPTER TWO: Literature review 5
2.1 The abuse of the naira note 6
2.2 Meat and its contamination 6
2.3 Characteristics of bacteria 8
CHAPTER THREE: Materials an method 20
3.1 Sample collection 20
3.2 Sterilization of materials 20
3.3 Media used and their preparation 20
3.4 Examination of currencies for contamination by Microorganism 21
3.5 Microbiological analysis 22
3.5.1 Macroscopic Examination 22
3.5.2 Microscopic Examination 22
3.5.3 Motility test 23
3.6 Biochemical test 23
3.6.1Oxidase 24
3.6.2 Catalase test 24
3.6.3 Coagulase test 24
3.6.4 Indole test 24
3.6.5 Fermentation test 25
3.7 Antibiotic sensitivity test 25
3.8 Statistical analysis 26
CHAPTER FOUR: Results 27
CHAPTER FIVE: Discussion, Conclusion and Recommendation 32
5.0 Discussion 32
5.1 Conclusion 36
5.3 Recommendation 36
References 38
Appendix 46
PHILIP, P (2020). ISOLATION AND IDENTIFICATION OF MICROORGANISM FROM NAIRA NOTES ASSOCIATED WITH MEAT SELLERS IN ARIARIA INTERNATIONAL MARKET ABA. Mouau.afribary.org: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-microorganism-from-naira-notes-associated-with-meat-sellers-in-ariaria-international-market-aba
PHILIP, PHILIP. "ISOLATION AND IDENTIFICATION OF MICROORGANISM FROM NAIRA NOTES ASSOCIATED WITH MEAT SELLERS IN ARIARIA INTERNATIONAL MARKET ABA" Mouau.afribary.org. Mouau.afribary.org, 29 Apr. 2020, https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-microorganism-from-naira-notes-associated-with-meat-sellers-in-ariaria-international-market-aba. Accessed 24 Nov. 2024.
PHILIP, PHILIP. "ISOLATION AND IDENTIFICATION OF MICROORGANISM FROM NAIRA NOTES ASSOCIATED WITH MEAT SELLERS IN ARIARIA INTERNATIONAL MARKET ABA". Mouau.afribary.org, Mouau.afribary.org, 29 Apr. 2020. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-microorganism-from-naira-notes-associated-with-meat-sellers-in-ariaria-international-market-aba >.
PHILIP, PHILIP. "ISOLATION AND IDENTIFICATION OF MICROORGANISM FROM NAIRA NOTES ASSOCIATED WITH MEAT SELLERS IN ARIARIA INTERNATIONAL MARKET ABA" Mouau.afribary.org (2020). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-microorganism-from-naira-notes-associated-with-meat-sellers-in-ariaria-international-market-aba