Isolation and identification of bacteria from diesel contaminated soils in Michael Okpara University of agriculture Umudike.
ABSTRACT
Isolation
and identification of bacteria from diesel contaminated soil was carried out
using Nutrient agar for total heterotrophic counts and mineral salt agar for
diesel degrading bacteria by vapor phase transfer technique. The highest mean
of total heterotrophic count was 3.8x10° cfu/g from uncontaminated soil and
least mean count 2.5x10° cfu/g from diesel contaminated soil there was no statistical
difference (P>0.05) in the total heterotrophic count of samples. The highest
mean count of diesel degrading bacteria was 3.4x10° cfu/g while the least count
was 2.0x10° for the control. There was significant difference in the mean count
of diesel degrading bacteria (P<0.05). The diesel degrading bacteria
isolated were of genera the genera identified were Bacillus sp, Staphylococcus
sp, Micrococcus sp, Corynebacterium sp, Alicagenes Sp, Flavobacterium sp,
Pseudomonas sp, Arthrobacter sp, Acinteobacter sp, Streptococcus sp, and
Klebsiella sp. These organisms were screened for their ability the degrade
diesel. All the organisms were able to grow in diesel at different rates with
Pseudomonas sp, Bacillus sp and Micrococcus sp showing heavy growths. These
bacteria isolated can be employed as seeds for bioremediation.
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IWEHA, I (2022). Isolation and identification of bacteria from diesel contaminated soils in Michael Okpara University of agriculture Umudike.. Mouau.afribary.org: Retrieved Nov 14, 2024, from https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-bacteria-from-diesel-contaminated-soils-in-michael-okpara-university-of-agriculture-umudike-7-2
IWEHA, IWEHA. "Isolation and identification of bacteria from diesel contaminated soils in Michael Okpara University of agriculture Umudike." Mouau.afribary.org. Mouau.afribary.org, 14 Dec. 2022, https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-bacteria-from-diesel-contaminated-soils-in-michael-okpara-university-of-agriculture-umudike-7-2. Accessed 14 Nov. 2024.
IWEHA, IWEHA. "Isolation and identification of bacteria from diesel contaminated soils in Michael Okpara University of agriculture Umudike.". Mouau.afribary.org, Mouau.afribary.org, 14 Dec. 2022. Web. 14 Nov. 2024. < https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-bacteria-from-diesel-contaminated-soils-in-michael-okpara-university-of-agriculture-umudike-7-2 >.
IWEHA, IWEHA. "Isolation and identification of bacteria from diesel contaminated soils in Michael Okpara University of agriculture Umudike." Mouau.afribary.org (2022). Accessed 14 Nov. 2024. https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-bacteria-from-diesel-contaminated-soils-in-michael-okpara-university-of-agriculture-umudike-7-2