Invitro Inhibitory Effect Of Bacteriocin From Lactic Acid Bacteria From Ugba (Pentaclethra Macrophylla Benth) Against Carrrot (Daucus Carrota) Spoilage Bacteria
ABSTRACT
This work studied the in vitro inhibitory
effect of bacteriocin from lactic acid bacteria (LAB) U from ugba against
spoilage bacteria of carrot (Daucus carrota). Seven bacteria species were
isolated from randomly selected spoilt carrot tubers from five (5) locations.
The isolates and their respective occurrences Staphylococcus aureus (100%),
Pseudomonas spp (75%), Proteus spp (40°). Serratia spp (80%). Bacillus spp
(80%), Corynebacterium spp (15.3) and Escherichia coil (40%). Two lactic acid
bacteria, Lactobacillus fermenti and Lactobacillus plantarum were isolated from
Ugba and their crude bacteriocin extracts were used for tests. Result of
pathogenecity test of' the spoilt carrot bacteria isolates, showed only three
isolates. Corynubacterium, Pseudomonas and Serratia species were confirmed to
be pathogens of fresh healthy carrot tubers. The inhibitory effects of the
crude bacteriocin extracts of' the lactic acid bacteria isolates showed
inhibitions of varied sizes against the pathogens in vitro. L. easei crude extracts
generated inhibition of zones of diameters in the range of 11.67mm
(Corynebacterium) to 13.67mm (Serratia) while L. fermenti cause inhibitions of
1 0.67mm Pseudomonas) to 12.67mm (Serratia). The inhibitory of L. planiarium
crude bacteriocin extract was in the range of' I 0.67mm (Corynebacterium) to
13.67mm (Serratia). The inhibitory activities of' the lactic acid bacteria
extracts were all lower than that of' a standard antibiotic (control) which
ranged between 18.67mm (Serratia) 22.0mm (Pseudomonas). The potentials of' the
crude bacteriocin extracts from lactic acid bacteria controlling carrot
spoilage bacteria was observed in spite of the relatively low level of
inhibitions.
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MICHAEL, U (2021). Invitro Inhibitory Effect Of Bacteriocin From Lactic Acid Bacteria From Ugba (Pentaclethra Macrophylla Benth) Against Carrrot (Daucus Carrota) Spoilage Bacteria. Mouau.afribary.org: Retrieved Nov 16, 2024, from https://repository.mouau.edu.ng/work/view/invitro-inhibitory-effect-of-bacteriocin-from-lactic-acid-bacteria-from-ugba-pentaclethra-macrophylla-benth-against-carrrot-daucus-carrota-spoilage-bacteria-7-2
UNIVERSITY, MICHAEL. "Invitro Inhibitory Effect Of Bacteriocin From Lactic Acid Bacteria From Ugba (Pentaclethra Macrophylla Benth) Against Carrrot (Daucus Carrota) Spoilage Bacteria" Mouau.afribary.org. Mouau.afribary.org, 19 Jul. 2021, https://repository.mouau.edu.ng/work/view/invitro-inhibitory-effect-of-bacteriocin-from-lactic-acid-bacteria-from-ugba-pentaclethra-macrophylla-benth-against-carrrot-daucus-carrota-spoilage-bacteria-7-2. Accessed 16 Nov. 2024.
UNIVERSITY, MICHAEL. "Invitro Inhibitory Effect Of Bacteriocin From Lactic Acid Bacteria From Ugba (Pentaclethra Macrophylla Benth) Against Carrrot (Daucus Carrota) Spoilage Bacteria". Mouau.afribary.org, Mouau.afribary.org, 19 Jul. 2021. Web. 16 Nov. 2024. < https://repository.mouau.edu.ng/work/view/invitro-inhibitory-effect-of-bacteriocin-from-lactic-acid-bacteria-from-ugba-pentaclethra-macrophylla-benth-against-carrrot-daucus-carrota-spoilage-bacteria-7-2 >.
UNIVERSITY, MICHAEL. "Invitro Inhibitory Effect Of Bacteriocin From Lactic Acid Bacteria From Ugba (Pentaclethra Macrophylla Benth) Against Carrrot (Daucus Carrota) Spoilage Bacteria" Mouau.afribary.org (2021). Accessed 16 Nov. 2024. https://repository.mouau.edu.ng/work/view/invitro-inhibitory-effect-of-bacteriocin-from-lactic-acid-bacteria-from-ugba-pentaclethra-macrophylla-benth-against-carrrot-daucus-carrota-spoilage-bacteria-7-2