Incidence Of Biofilm Formation And Methicillin Resistance Among Clinical Isolates Of Staphylococcusaureus Obtained From Mercy Specialist Hospital, Ahiaeke, Ndume, Abia State.
ABSTRACT
This study was carried out to
determine the incidence of bioflim formation and Methicillin resistance among
clinical isolates of Staphylococcus aureus obtained from Mercy Specialist
Hospital, Ahiaeke, Umuahia, Abia state. A total of 30 randomly selected
patients were included in the study. Clinical samples collected were subjected'
to standard bacteriological culture. S. aureus isolates were identified by
mannitol fermentation, coagulase positivity and catalase positivity.
Antimicrobial susceptibility test was performed on Mueller-hinton agar (MHA) by
modified Kirby-Bauer disc diffusion method. Biofilm producers were also
detected using the conga red agar method. Out of total 30 clinical samples, S.
aureus was isolated in 63.3% (n=19). Among the isolates, 63.1% (n=12) were from
male patients whereas 36.8% (n=7) were from female. There was no sex difference
in colonization of S. aureus. Out of 19 isolates, 68.4% (n=13) were MRSA. MRSA
isolates indicated relatively high rate of resistance to antibiotics
Ceftriaxone (84.6%) followed by Erythromycin and Ciprofloxacin with (76.9%)
each, then Cloxacillin (69.2%) and Ampicillin (61.5%) respectively. The
isolates showed the lowest resistance to Gentamicin (30.8%). Methicillin
resistance was determined using the Cefoxitin and Oxacillin disc diffusion
methods. Biofilm formation in Staphylococcus aureus isolates using the Congo
red agar (CRA) method could detect 10 (52.6%) of Staphylococcus aureus as
strong bioflim producers which include 9 (47.4%) MRSA, and 1 (5.3%) MSSA. This
study showed a slightly high incidence of biofilm formation and Methicillin
resistance among clinical isolates of Staphylococcus aureus obtained from Mercy
Specialist Hospital, Ahiaeke, Umuahia, Abia state.
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EKE, A (2021). Incidence Of Biofilm Formation And Methicillin Resistance Among Clinical Isolates Of Staphylococcusaureus Obtained From Mercy Specialist Hospital, Ahiaeke, Ndume, Abia State. . Mouau.afribary.org: Retrieved Nov 16, 2024, from https://repository.mouau.edu.ng/work/view/incidence-of-biofilm-formation-and-methicillin-resistance-among-clinical-isolates-of-staphylococcusaureus-obtained-from-mercy-specialist-hospital-ahiaeke-ndume-abia-state-7-2
AGBAEZE, EKE. "Incidence Of Biofilm Formation And Methicillin Resistance Among Clinical Isolates Of Staphylococcusaureus Obtained From Mercy Specialist Hospital, Ahiaeke, Ndume, Abia State. " Mouau.afribary.org. Mouau.afribary.org, 13 Jul. 2021, https://repository.mouau.edu.ng/work/view/incidence-of-biofilm-formation-and-methicillin-resistance-among-clinical-isolates-of-staphylococcusaureus-obtained-from-mercy-specialist-hospital-ahiaeke-ndume-abia-state-7-2. Accessed 16 Nov. 2024.
AGBAEZE, EKE. "Incidence Of Biofilm Formation And Methicillin Resistance Among Clinical Isolates Of Staphylococcusaureus Obtained From Mercy Specialist Hospital, Ahiaeke, Ndume, Abia State. ". Mouau.afribary.org, Mouau.afribary.org, 13 Jul. 2021. Web. 16 Nov. 2024. < https://repository.mouau.edu.ng/work/view/incidence-of-biofilm-formation-and-methicillin-resistance-among-clinical-isolates-of-staphylococcusaureus-obtained-from-mercy-specialist-hospital-ahiaeke-ndume-abia-state-7-2 >.
AGBAEZE, EKE. "Incidence Of Biofilm Formation And Methicillin Resistance Among Clinical Isolates Of Staphylococcusaureus Obtained From Mercy Specialist Hospital, Ahiaeke, Ndume, Abia State. " Mouau.afribary.org (2021). Accessed 16 Nov. 2024. https://repository.mouau.edu.ng/work/view/incidence-of-biofilm-formation-and-methicillin-resistance-among-clinical-isolates-of-staphylococcusaureus-obtained-from-mercy-specialist-hospital-ahiaeke-ndume-abia-state-7-2