ABSTRACT
This
study was conducted to examine the In vitro antifungal activity of leaf ethanol
extracts of Azadirachta indica, Senna alata, Eucalyptus camaldulensis and
Tithonia diversifolia against Phytophthora colocasiae Racib. Extracts were tested
against isolated pure culture of P. colocasiae at 5, 10 and 20mg/ml. 3mg/ml
Ridomil and untreated culture served as the standard and absolute control
respectively. Experiment was conducted in a completely Randomized Design (CRD)
and all treatments were replicated thrice. Data were subjected to analysis of
variance (ANOVA) using statistical package for social scientists (SPSS). Means
were separated by Duncan Multiple Range Test (DMRT) and test of significance at
5%. Results indicated that 20mg/ml extracts of A. indica and 3mg/ml of Ridomil
had the best percentage radial growth inhibition of P. colocasiae at 100%,
followed by S. alata (78.19%) at 5mg/ml while extract of E. camaldulensis
showed the lowest anti-fungal activity at 5mg/ml (19.33%). P. colocasiae growth
in absolute control was un-inhibited (0.00%). Mycelia dry weight of P.
colocasiae at lOmg/ml of E. camaldulensis was recorded at 0.10±0.61g contrary
to 0.00g recorded against other plant extracts. At 20mg/ml, A. indica supported
a mycelia dry weight of 0.09+0.06g but none was recorded against other plant
extracts. Ridomil did not support mycelia growth in PDB while absolute control
had a significant (p<0.05) mycelia dry weight of 0.27+0.08g. Leaf extract
ofA. indica should be characterized to obtain the pure active component
responsible for anti-fungal activity and used for further studies in the
control ofthe pathogen in the field.
CHINWEOKWU, R (2024). In vitro Evaluation Of Selected Plant Extracts Against Phytophthora Colocasiae Racib. Causing Taro Leaf Blight (TLB):- Ehumadu, Chinweokwu R.. Mouau.afribary.org: Retrieved Sep 08, 2024, from https://repository.mouau.edu.ng/work/view/in-vitro-evaluation-of-selected-plant-extracts-against-phytophthora-colocasiae-racib-causing-taro-leaf-blight-tlb-ehumadu-chinweokwu-r-7-2
R, CHINWEOKWU. "In vitro Evaluation Of Selected Plant Extracts Against Phytophthora Colocasiae Racib. Causing Taro Leaf Blight (TLB):- Ehumadu, Chinweokwu R." Mouau.afribary.org. Mouau.afribary.org, 13 Mar. 2024, https://repository.mouau.edu.ng/work/view/in-vitro-evaluation-of-selected-plant-extracts-against-phytophthora-colocasiae-racib-causing-taro-leaf-blight-tlb-ehumadu-chinweokwu-r-7-2. Accessed 08 Sep. 2024.
R, CHINWEOKWU. "In vitro Evaluation Of Selected Plant Extracts Against Phytophthora Colocasiae Racib. Causing Taro Leaf Blight (TLB):- Ehumadu, Chinweokwu R.". Mouau.afribary.org, Mouau.afribary.org, 13 Mar. 2024. Web. 08 Sep. 2024. < https://repository.mouau.edu.ng/work/view/in-vitro-evaluation-of-selected-plant-extracts-against-phytophthora-colocasiae-racib-causing-taro-leaf-blight-tlb-ehumadu-chinweokwu-r-7-2 >.
R, CHINWEOKWU. "In vitro Evaluation Of Selected Plant Extracts Against Phytophthora Colocasiae Racib. Causing Taro Leaf Blight (TLB):- Ehumadu, Chinweokwu R." Mouau.afribary.org (2024). Accessed 08 Sep. 2024. https://repository.mouau.edu.ng/work/view/in-vitro-evaluation-of-selected-plant-extracts-against-phytophthora-colocasiae-racib-causing-taro-leaf-blight-tlb-ehumadu-chinweokwu-r-7-2