ABSTRACT

This study was used to determine the microbial implication of biobased fluid. Lard used in this was processed after been purchase from local sellers within Umuahia metropolis. After the samples were cultured on solid media using pour plate techniques. The bacteria species were identified as Staphylococcus aureus, Escherichia coli and Salmonella species while the fungi species include Aspergillus niger and Aspergillus fiavus. The Total Viable Staphylococcus aureus ranges from l.01x105and 1.32x105cfu/ml respectively. While the total fungal count (TFC) Aspergillus niger and Aspergillusfiavus of organism suspension at day 0, day 3 and day 6 range from 0.34x105and 0.64x i05cfulml, the pH of each of the bacterial organism suspension at day 0, day 3 and day 6 incubation ranges from 7. 00 and 7.41 respectively are seen in Table 4.4a. while the pH of each of the fungal organism suspension at day 0, day 3 and day 6 incubation were 7.12 to 7. 22 respectively. The Optical Density of each of the bacterial organism suspension at day 0, day 3 and day 6 incubation 0.267 to 1.53 irespectively. The physicochemical parameter of hydraulic fluid. From the result viscosity at 30°c.