ABSTRACT

 The prevalence of Gram-Negative bacteria from urine samples and their susceptibility to various antibiotics were investigated. In this study, a total of five (5) Gram Negative bacteria species were isolated and identified by their morphological characteristics, pigmentation on media, microscopy, biochemical and sugar fermentation methods. The bacterial species isolated include; Escherichia co/i, Kiebsiella spp, Pseudomonas spp, Proteus spp and Enterobacter spp respectively. The present study revealed that the distribution and percentage occurrence of Gram Negative bacterial isolates from the urine specimens were different. In the urine specimens from female individuals, the predominant bacterial isolates were Escherichia co/i 25(27.5%), while in male individuals the predominant bacterial isolates were Escherichia co/i 30(35.7%). It was also revealed in this study that the antibiotic susceptibility and resistant profile for Gram Negative isolates varied. Escherichia co/i and Pseudomonas spp showed high level of sensitive to the antibiotics Levofloxacin (2Omcg), Ciprofloxacin (lOmcg) and Gentamycin (lOmcg). It was also recorded that Amoxil (2Orncg), Streptomycin (30mcg), Erythromycin (3omcg), and Ampiclox (20mcg) antibiotics showed appreciable effect against Gram Negative bacteria isolates (Escherichia co/i and Pseudomonas spp). The resistant profile of the gram negative isolates revealed that Escherichia co/i and Pseudomonas spp had highest number of isolates resistant to the antibiotics Chloramphenicol (30mcg) and Norfioxacin (lOmcg), This implies that these antibiotics cannot be used as empirical therapy for urinary tract infection particularly in the study area. However, Gentarnicin (lOrncg), Ciprofloxacin (lOmcg) and Levofloxacin (20mcg) antibiotic could be of alternative of choice to use and to control Gram Negative bacterial infection as an effective antibacterial agent.