Extraction of bromelain from pineapple crown and fruit and effect of pH on its activity:- Madu, Adanna B.
ABSTRACT
Pineapple is
one of the common fruits in Nigeria, with an edible flesh while the crown, peel
and leaves are considered wastes and discarded or littered on the environment.
This study assessed the enzymatic activity of crude bromelain extracts from
pineapple crown and fruit using ammonium sulphate precipitation method.
Phosphate buffer of varying pH ranges (4.6, 5.8, 7.0 and 8.2) was used to
extract crude bromelain while casein was used as the substrate. The
experimental result was compared to a known standard (Tyrosine) and the results
were positive. The concentrations of the crude bromelain extract from the crown
were 77.43 ± 0.61, 77.7 ± 0.10, 198.64 ± 38.28 and 1082.86 ± 00 µg/ml, while
that of the fruit was 99.57 ± 0.00, 142.22 ± 0.11, 235.29 ± 0.71 and 752.86 ±
1.01 µg/ml for pH ranges 4.6, 5.8, 7.0 and 8.2 respectively. The enzyme
activity of the crown sample obtained from this study was 0.775, 0.772, 1.712
and 10.831 TU/ml, while that of the fruit was 0.996, 1.423, 2.353 and 7.530
TU/ml. The result showed that the enzyme activity of the crude bromelain
extracts from pineapple crown and fruit increased with increase in pH value.
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ADANNA, B (2023). Extraction of bromelain from pineapple crown and fruit and effect of pH on its activity:- Madu, Adanna B.. Mouau.afribary.org: Retrieved Nov 17, 2024, from https://repository.mouau.edu.ng/work/view/extraction-of-bromelain-from-pineapple-crown-and-fruit-and-effect-of-ph-on-its-activity-madu-adanna-b-7-2
BERNEDETH, ADANNA. "Extraction of bromelain from pineapple crown and fruit and effect of pH on its activity:- Madu, Adanna B." Mouau.afribary.org. Mouau.afribary.org, 13 Nov. 2023, https://repository.mouau.edu.ng/work/view/extraction-of-bromelain-from-pineapple-crown-and-fruit-and-effect-of-ph-on-its-activity-madu-adanna-b-7-2. Accessed 17 Nov. 2024.
BERNEDETH, ADANNA. "Extraction of bromelain from pineapple crown and fruit and effect of pH on its activity:- Madu, Adanna B.". Mouau.afribary.org, Mouau.afribary.org, 13 Nov. 2023. Web. 17 Nov. 2024. < https://repository.mouau.edu.ng/work/view/extraction-of-bromelain-from-pineapple-crown-and-fruit-and-effect-of-ph-on-its-activity-madu-adanna-b-7-2 >.
BERNEDETH, ADANNA. "Extraction of bromelain from pineapple crown and fruit and effect of pH on its activity:- Madu, Adanna B." Mouau.afribary.org (2023). Accessed 17 Nov. 2024. https://repository.mouau.edu.ng/work/view/extraction-of-bromelain-from-pineapple-crown-and-fruit-and-effect-of-ph-on-its-activity-madu-adanna-b-7-2