ABSTRACT
Isolation and identification of Pseudomonas aeruginosa from Hospital environment for pyocyanin production was done. The
isolates from Hospital bed and wound swab were identified as Pseudomonas
aeruginosa. The virulence factor,
antibiotic pigment, pyocyanin which is one of the characteristic feature of P. aeruginosa and which also confers on
it growth advantage over competing bacteria occupying the same niche was also
isolated and characterised. Quantitative assay of pyocyanin production
was conducted which is based on the absorbance of pyocyanin at 520 nm in acidic
solution. The concentration of pyocyanin was determined by multiplying the
optical density at 520 nm (OD520) by 17.072. The result showed that the
isolates produced pyocyanin with the total concentration of 13μg/ml, while the
isolates from wound swab produced no pyocyanin but pyorubin. Pyocyanin produced
was characterized by biochemical tests, its dissolution in chloroform and
Hydrochloric acid (HCl) to give a blue and pink to red colour respectively
confirms the presence of pyocyanin. The
purification of pyocyanin was done by liquid-liquid extraction method using
Na-borate buffer at the pH of 10 to neutralize the HCl and re extracted into
chloroform and acid before storage. Since pyocyanin is labile to many
conditions, the purified pyocyanin was stored in acid at freezing temperature
in dark container to prevent decomposition. Since pyocyanin can be isolated
successfully from P. aeruginosa, it
can be used in vitro to carry out its antimicrobial activity on the growth of
other microorganisms; also study on the mechanism of its virulence can be done
using the isolated pyocyanin.
ANENEH, A (2022). Extraction And Purification Of Pyocyanin From Pseudomonas Aeruginosa Isolated From Hospital Environment. Mouau.afribary.org: Retrieved Nov 13, 2024, from https://repository.mouau.edu.ng/work/view/extraction-and-purification-of-pyocyanin-from-pseudomonas-aeruginosa-isolated-from-hospital-environment-7-2
ANENEH, ANENEH. "Extraction And Purification Of Pyocyanin From Pseudomonas Aeruginosa Isolated From Hospital Environment" Mouau.afribary.org. Mouau.afribary.org, 16 Dec. 2022, https://repository.mouau.edu.ng/work/view/extraction-and-purification-of-pyocyanin-from-pseudomonas-aeruginosa-isolated-from-hospital-environment-7-2. Accessed 13 Nov. 2024.
ANENEH, ANENEH. "Extraction And Purification Of Pyocyanin From Pseudomonas Aeruginosa Isolated From Hospital Environment". Mouau.afribary.org, Mouau.afribary.org, 16 Dec. 2022. Web. 13 Nov. 2024. < https://repository.mouau.edu.ng/work/view/extraction-and-purification-of-pyocyanin-from-pseudomonas-aeruginosa-isolated-from-hospital-environment-7-2 >.
ANENEH, ANENEH. "Extraction And Purification Of Pyocyanin From Pseudomonas Aeruginosa Isolated From Hospital Environment" Mouau.afribary.org (2022). Accessed 13 Nov. 2024. https://repository.mouau.edu.ng/work/view/extraction-and-purification-of-pyocyanin-from-pseudomonas-aeruginosa-isolated-from-hospital-environment-7-2