ABSTRACT
The aim of this study is to determine
microorganisms found in the Ear, Throat and Nose of Michael Okpara Unversity of
Agriculture .students. A total of 30 swab samples of throat swab, nasal (nose)
swab, and aural (ear) swab were collected aseptically using sterile Evepon swab
sticks and the sample collected were properly labeled accordingly,. The swabs
were immediately transported in sterile cotton plugged test tube to the
Microbiology laboratory for further analysis. Throat swab were inoculated on
chocolate agar plates, whereas nasal (nose) and ear swab were inoculated on
Chocolate agar, Nutrient agar and MacConkey agar plates. All the plates were
incubated for 24 hours aerobically with the exception of chocolate agar plate
that was incubated in a candle jar. The plates were examined for the growth of
bacteria and the colonies were identified by conventional methods. Haemolysis
was studied on chocolate agar plates. Discrete and pure colonies of bacterial
isolates were stored instantly in the refrigerator until they were required for
identification tests and incubated at 37°C for 24hours. A total number of six
(6) bacterial genera {Escherichia coli, Staphylococcus aureus, Pseudomonas
aeruginosa, Streptococcus pyogenes, Klebsiella pneumoniae and Proteus vulgaris)
were isolated from Ear, Nose and Throat ofstudents in Michael Okpara University
ofAgriculture Umudike, Abia State . Growth was observed in all the samples
(100%) as all cultures were positive with 41 isolates. Pseudomonas aeruginosa
had (29%), Staphylococcus aureus (24.4%), Klebsiella pneumoniae (19%),
Escherichia coli (12%) and Proteus vulgaris (5%) . The ear swabs was positive
in 26 (29.21%) male and 24(26.96%) in female. Throat swabs was positive in 13
(14.60%) male and 12 (13.48%) in female, while in nose swabs 6 (6.74%) was
positive in male and 8 (8.98%) in female . The most predominant organism in
distribution was Klebsiella pneumoniae (24%), followed by Streptococcus
pyogenes (20%), Staphylococcus aureus (17%), Proteus vulgaris (17%), while the
least in occurrence were Escherichia coli (12%) and Pseudomonas aeruginosa
(12%). {Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa,
Streptococcus pyogenes, Klebsiella pneumonia and Proteus vulgaris are the
leading Ear, Nose and Throat pathogens in Students of Micahel Okpara University
of Agriculture, Umudike. Most of the isolates showed high prevalent rates.
However, the judicious use of antibiotics should be considered for the better
treatment and to avoid the development ofresistant organisms in Ear, Nose and
Throat patients and thus recommended that Diagnosis of infection should not be
made on clinical diagnosis only, it should be confirmed by laboratory diagnosis
MICHAEL, U (2023). Evaluation of The Microbial Flora Of The Ear Nose And Throat Of Mouau Students :- Udeh, Blessing N.. Mouau.afribary.org: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/evaluation-of-the-microbial-flora-of-the-ear-nose-and-throat-of-mouau-students-udeh-blessing-n-7-2
UNIVERSITY, MICHAEL. "Evaluation of The Microbial Flora Of The Ear Nose And Throat Of Mouau Students :- Udeh, Blessing N." Mouau.afribary.org. Mouau.afribary.org, 19 Oct. 2023, https://repository.mouau.edu.ng/work/view/evaluation-of-the-microbial-flora-of-the-ear-nose-and-throat-of-mouau-students-udeh-blessing-n-7-2. Accessed 24 Nov. 2024.
UNIVERSITY, MICHAEL. "Evaluation of The Microbial Flora Of The Ear Nose And Throat Of Mouau Students :- Udeh, Blessing N.". Mouau.afribary.org, Mouau.afribary.org, 19 Oct. 2023. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/evaluation-of-the-microbial-flora-of-the-ear-nose-and-throat-of-mouau-students-udeh-blessing-n-7-2 >.
UNIVERSITY, MICHAEL. "Evaluation of The Microbial Flora Of The Ear Nose And Throat Of Mouau Students :- Udeh, Blessing N." Mouau.afribary.org (2023). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/evaluation-of-the-microbial-flora-of-the-ear-nose-and-throat-of-mouau-students-udeh-blessing-n-7-2