Determination Of The Cyanogenic Potential Of Cassava, Partial Purification And Hydrolytic Activity Of B-Glucosidase From Two Cultivars Of Cassava (505 And 419)
ABSTRACT
B-glucosidse was partially purified
from plant source (cassava — 505 and 419 cultivars) by extraction with acetate
buffer, separate fractional precipitation with ammonium sulfate and acetone
followed by heat treatment. The content of partially purified enzyme from each
cultivar gave 0.46ing/ml 0.069mg/mi respectively. Mathematical treatment of the
data from the degradation of linamarin by the partially purified enzyme generated HCN values that were used to construct line weaver Burk plot which gave
apparent km and values of 5.75mm and 3.2x102mmo1 HCNmin/ml for 505
cultivars, and 7.80mm and 3.7x102mmo1 HCN/min/mi for 1 19 cultivar. The temperature
and PH optima obtained for both the crude and partially purified enzyme were
450C — 500c and 6.0 — 6.5. Both the crude and partially purified enzyme showed
high degree of hydrolysis towards standard linamarin and cyanogenic glucosides
of cassava.
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NWOKO, S (2021). Determination Of The Cyanogenic Potential Of Cassava, Partial Purification And Hydrolytic Activity Of B-Glucosidase From Two Cultivars Of Cassava (505 And 419). Mouau.afribary.org: Retrieved Nov 18, 2024, from https://repository.mouau.edu.ng/work/view/determination-of-the-cyanogenic-potential-of-cassava-partial-purification-and-hydrolytic-activity-of-b-glucosidase-from-two-cultivars-of-cassava-505-and-419-7-2
S., NWOKO. "Determination Of The Cyanogenic Potential Of Cassava, Partial Purification And Hydrolytic Activity Of B-Glucosidase From Two Cultivars Of Cassava (505 And 419)" Mouau.afribary.org. Mouau.afribary.org, 23 Jul. 2021, https://repository.mouau.edu.ng/work/view/determination-of-the-cyanogenic-potential-of-cassava-partial-purification-and-hydrolytic-activity-of-b-glucosidase-from-two-cultivars-of-cassava-505-and-419-7-2. Accessed 18 Nov. 2024.
S., NWOKO. "Determination Of The Cyanogenic Potential Of Cassava, Partial Purification And Hydrolytic Activity Of B-Glucosidase From Two Cultivars Of Cassava (505 And 419)". Mouau.afribary.org, Mouau.afribary.org, 23 Jul. 2021. Web. 18 Nov. 2024. < https://repository.mouau.edu.ng/work/view/determination-of-the-cyanogenic-potential-of-cassava-partial-purification-and-hydrolytic-activity-of-b-glucosidase-from-two-cultivars-of-cassava-505-and-419-7-2 >.
S., NWOKO. "Determination Of The Cyanogenic Potential Of Cassava, Partial Purification And Hydrolytic Activity Of B-Glucosidase From Two Cultivars Of Cassava (505 And 419)" Mouau.afribary.org (2021). Accessed 18 Nov. 2024. https://repository.mouau.edu.ng/work/view/determination-of-the-cyanogenic-potential-of-cassava-partial-purification-and-hydrolytic-activity-of-b-glucosidase-from-two-cultivars-of-cassava-505-and-419-7-2