Determination Of Cyanogenic Potential Of Some CMD Cassava Cultivars And Partial Purification And Some Propertities Of The Β-Glucosidase.
ABSTRACT
The determination of cyanogenic potential of some CMD
cassava cultivars as well as partial purification and some properties of their
f3- glucosidase were carried out using biochemical and spectrophotometric
methods. The cyanogenic potential determined for peels, pulp and leaves of
TMS/98/0581 were52.8, 94.40 and 122.0 mgCNkg -1 and 72.3, 174.2 and 273
mgCNkg-1 for TMS/98/0510 respectively. Partial purification of the
f3-glucosidase from these cultivars by ammonium sulphate precipitation followed
by centrifugation and dialysis yielded 3-glucosidase with activity of4.42units/ml
for TMS/98/0581 cultivar and 2.5 units/mSl for TMS/98/0510.The Km and Vmax
values determined for these enzymes using line-weaver Burk plot and cyanide
yield from linamarin as substantiated were 2.78 and 2.33pm01 for S95, 2.78 and
3.57pm01 for TMS/98/0510.
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MICHAEL, U (2021). Determination Of Cyanogenic Potential Of Some CMD Cassava Cultivars And Partial Purification And Some Propertities Of The Β-Glucosidase.. Mouau.afribary.org: Retrieved Nov 18, 2024, from https://repository.mouau.edu.ng/work/view/determination-of-cyanogenic-potential-of-some-cmd-cassava-cultivars-and-partial-purification-and-some-propertities-of-the-glucosidase-7-2
UNIVERSITY, MICHAEL. "Determination Of Cyanogenic Potential Of Some CMD Cassava Cultivars And Partial Purification And Some Propertities Of The Β-Glucosidase." Mouau.afribary.org. Mouau.afribary.org, 16 Jul. 2021, https://repository.mouau.edu.ng/work/view/determination-of-cyanogenic-potential-of-some-cmd-cassava-cultivars-and-partial-purification-and-some-propertities-of-the-glucosidase-7-2. Accessed 18 Nov. 2024.
UNIVERSITY, MICHAEL. "Determination Of Cyanogenic Potential Of Some CMD Cassava Cultivars And Partial Purification And Some Propertities Of The Β-Glucosidase.". Mouau.afribary.org, Mouau.afribary.org, 16 Jul. 2021. Web. 18 Nov. 2024. < https://repository.mouau.edu.ng/work/view/determination-of-cyanogenic-potential-of-some-cmd-cassava-cultivars-and-partial-purification-and-some-propertities-of-the-glucosidase-7-2 >.
UNIVERSITY, MICHAEL. "Determination Of Cyanogenic Potential Of Some CMD Cassava Cultivars And Partial Purification And Some Propertities Of The Β-Glucosidase." Mouau.afribary.org (2021). Accessed 18 Nov. 2024. https://repository.mouau.edu.ng/work/view/determination-of-cyanogenic-potential-of-some-cmd-cassava-cultivars-and-partial-purification-and-some-propertities-of-the-glucosidase-7-2